Hi good people here,
I have a haploid genome (phase)for 40 individuals
1. individual1.haploid1.fasta + its index file 2. individual2.haploid1.fasta + its index file : : : : 40. individual40.haploid40.fasta + index file
Please can you advise
- how to call variants and which are the best tools?
- is it necessary to align each fasta file before calling variants?
Apology, I know my questions are too basic but please please pardon me
Thank you very much