I have this command:
blastn -db /home/BLAST_nt/nt -query otu_greedy_0.97_82.fasta -outfmt 5 -out otu_greedy_0.97_82_blastn.xml -evalue 0.001
And i have to include the following conditions:
percent identity of 95%, e-value of 0.001, minimum query coverage of 100%, best hit score edge of 0.05 and best hit overhang of 0.25.
should I include these conditions in the code above? if yes, how can i do that?