m6A-seq input normalisation with RNA-seq
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3 months ago

I have an m6A-seq dataset for a cell type only with IP treatment alone(Without input).But they have performed RNA-seq for that cell type and processed as following

"m6A peaks were identified by the comparison of the read abundance between m6A-seq and RNA-seq samples".

For my analysis I need to generate an input normalised m6A coverage enrichment file(Bigwig file) using bamCompare of deepTools after mapping the m6A seq and RNA-seq data of the cell type.

Is it fine to use RNA-seq as an input and ????

thanks in advance

MeRIP-Seq bamCompare deepTools m6A-seq normalisation • 182 views

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