Cellchat error: data.signaling matrix error when using mouse database (Error in identifyOverExpressedGenes)
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Entering edit mode
10 months ago
Aydin • 0

Hi, I'm having an error similar to this cellchat user (https://github.com/sqjin/CellChat/issues/264). If I run CellChatDB.mouse, and I subset my cellchat object (subsetData), I get this error:

Error in identifyOverExpressedGenes(cellchat_DMSO) : 
  Please check `object@data.signaling` and ensure that you have run `subsetData` and that the data matrix `object@data.signaling` looks OK

When I look at the data.signalling matrix of my cellchat object, I see 2 rows:

> cellchat_DMSO@data.signaling
2 x 10547 sparse Matrix of class "dgCMatrix"
  [[ suppressing 66 column names ‘AAACCCAAGAATTCAG-1’, ‘AAACCCACAACCCTCT-1’, ‘AAACCCACACCAGCCA-1’ ... ]]

C3 . . . . . . . . . .        . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2.183826 . . . . . .
F2 . . . . . . . . . 2.642966 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .        . . . . . .

C3 . .        ......
F2 . 0.943755 ......

 .....suppressing 10481 columns in show(); maybe adjust 'options(max.print= *, width = *)'
 ..............................

I do not get this same error when I use CellChatDB.human. How can I fix my data.signalling matrix? For reference, my cellchat object came from Seurat input.

Thank you for your help!

Seurat Cellchat scRNA • 783 views
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