I am looking for information regarding the unit of the puck coordinates in slideseq data.
In the last section of the Puck Sequencing part in the 2019 science paper (doi: 10.1126/science.aaw1219), I see the following line.
The final stitched images were 6030 pixels by 6030 pixels.
My concern is I have been trying to combine multiple datasets. I see some even have negative X, Y coordinate which suggest that is centered.
1) What is the unit of the X, Y coordinate (Pixels or microns?)
2) What is a good way to combine multiple datasets without worrying about the coordinate scale differences. (For instance, In Visium data, scale.json file contains the pixels covered each spot that allows swapping all of the coordinate values to microns.)
Its been so long since I asked a new question. I am once again asking for your wisdom.