Estimating sample size from bulk RNAseq data
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9 months ago
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Is it possible to estimate the sample size (number of cells) in a bulk RNASeq sample data?

rnaseq samplesize sequencing • 477 views
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You may find an estimation of cellular type composition, with deconvolution approaches. It's barely impossible to assess the absolute number of all the cells in your bulk.

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Its hard to say how many cells was used in a Bulk RNA-SEQ, because we ignore the cells identity and extract RNA from whole cells.

Maybe you can try a control gene in the sample as housekeeping and count the number isoform/gene seen in the sample as the cell numbers, but its hard.

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