Hi, I met a question when I analyzed scRNA-seq data. I found a cluster expresses the markers of myeloid cells and B cells. And in this cluster, cells expressing myeloid marker did not overlap with cells expressing B cell marker. The images as follows:
The markers of myeloid cells:
The markers of B cells:
And I think the cluster 10 is not doublet because its nCount_RNA and nFeature_RNA are normal.
So why does this happen, and what should I do to annotate this cluster.
Can anyone help me or give me some advice?