I have a control with another technical replicate then I try to down load a biological replicate to make the statistics more robust. I looked at the raw count and there is big different between the biological replicate and the two technical replicates. Could I do keep that 3 controls for down stream analysis or I need normalization with the biological replicate before adding to the two technical replicate. The PCA bellow has 3 diseased samples with 2 technical replicates each. The control I add even closer with the diseased than the first control. I appreciate your help! Unfortunately, I don't know anyone else near me who can help. They are just busy with their work.