I'm trying to use my self-developed tool to test it on long-read and short-read single-cell RNA-seq datasets. To ensure a fair comparison, I'm attempting to simulate short-length single-cell RNA-seq data from the long-read RNA-seq data. For example, for each sequence in the long-read FASTQ file, I will randomly select sequences that are 100 bp long to simulate a Smart-seq dataset. I will then use my tool for comparison. I'm wondering if there is a tool that can perform a similar task. I've noticed some people using Polyester to achieve this with transcript datasets, but I'm curious if I can directly use the FASTQ data to simulate short-read RNA-seq.