Hello I need your help with my analysis.
So i have an bulk Rna-seq dataset which contains data from ips cells which develop into neurons in 5 days For each time point I have 7 replicates
So now with deseq2 I have made all the pair-wise comparisons and I want to continue by comparing each time point to all the others (e.g time point 1 vs all the other time points) in order to find specific genes for each developmental stage.
I have seen several ways online to do so but I am a bit confused.
Any ideas how t build these kind of contrasts ?
Thanks in advance, A