Deleted:issue in RNA -seq analysis
0
0
Entering edit mode
12 weeks ago
subhiksha ▴ 30

hello all. i am working on RNA seq analysis. i would like to know following things: first i downloaded genome fasta file for non-coding rna from ensembl and got the gtf file for hg38 from there itself. performed hist2 and got 17% alignment for my sample against ncrna genome. now i sorted the file and then ran feature counts with the hg38 gtf file. but got zero counts, where is the issue.

also i would like to know. my bam file after sorting has the SRR id at first whereas the GTF file has chromosome no: . can this be a issue?

NGS RNA-Seq • 380 views
ADD COMMENT
This thread is not open. No new answers may be added
Traffic: 1574 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6