GO, KEGG, GSEA analysis with ~5 DEGs
1
0
Entering edit mode
10 weeks ago
foejvs546 ▴ 10

Hi friends,

Does it make sense to do pathway analysis if there are only 5 DEGs?

Thanks!

GO KEGG • 424 views
ADD COMMENT
0
Entering edit mode

Which is the experimental design?

ADD REPLY
0
Entering edit mode

Hi Shred ,

Treated vs untreated cells, 3 replicates each

ADD REPLY
1
Entering edit mode
10 weeks ago

If multiple genes indicate functions that should not occur multiple times by chance alone then that is the answer.

A single gene can provide functional interpretation after all.

But you are correct in being cautious - when you have few genes, the answers can be quite unreliable.

ADD COMMENT

Login before adding your answer.

Traffic: 1339 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6