Hello

I was curious how would unequal sample sizes of groups (e.g. healthy, 3 subjects and disease, 6 subjects), influence differential expression and differential abundance of celltypes?

E.g. in this case, given that disease is 2x of healthy in number of patients-

- How would this affect the differential expression of cell type A in healthy vs disease?
- Does differential abundance accounts for this inequality in sample size? and should we account for it in the DE for step 1?