Cell communacations analysis. I only have the proteome of one type of cell

Question

[David Arturo]

Good day, everybody!

I am just starting in bioinformatics and I would like to do a cell to cell communication analysis. I know there are various tools for that purpose such as Cellchat, Cellphonedb, Nichenet, etcetera. However, I see that every one of those commonly has ScRNAseq data as input.

Right now I only have a proteome of a cell culture with only one cell type. Would it be aproppiate to input the differential expression of such data set to a cell to cell communication analysis tool? If so... Which one and Why would that be the case?

Thank you. Best wishes.

[ATpoint]

If you want to infer communication then you need at least one sender and one receiver. How would that go with just one single bulk sample?

[QX]

Hi ATpoint, I wonder if it makes any sense to perform kind of autocrine signaling analysis; that splitting the one-cell-type dataset randomly then performing nichenet?