POD5 files
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6 weeks ago
marco.barr ▴ 130

Hello everyone, I'm wondering if there's a way to extract the reads located on the positive strand from pod5 files. Which commands among pod5 subset, filter from pod5 tool can I use? Or should I work directly from the fastq files? Thanks for your help.

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If you know which strand is "positive" then you should be able to get the reads from alignments using something like Samtools View: Only Forward Or Reverse Strand

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