Polish a large genome with Pilon
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7 days ago
sansan_96 ▴ 90

Hello everyone,

I'm trying to polish a large genome (3Gb) with Pilon but I'm having problems with RAM. I read that some people choose to split the genome to deal with RAM, so I would like to try this alternative. However, I have a question about how I can separate the genome and finally join the outputs of each independent polish. Does anyone have experience with this?. My genome is somewhat fragmented (10,000 scaffolds).

I would appreciate any comments.

Pilon Pacbio Polish Illumina • 155 views
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