Entering edit mode
3 months ago
bioinfo
▴
150
Hello,
I created an genome for IGV using a json file like shown below. I created one for both human and mouse.
{
"id": "humanv111",
"name": "humanv111",
"fastaURL": "E:\\Homo_sapiens.GRCh38.111.dna.primary_assembly.fa",
"indexURL": "E:\\Homo_sapiens.GRCh38.dna.primary_assembly.fa.fai",
"tracks": [
{
"name": "Genes",
"type": "annotation",
"url": "E:\\Homo_sapiens.GRCh38.111.sorted.gtf",
"indexed": false,
"searchable": true
}
] }
For the mouse once I import it it says that it cannot find an index and I can press cancel and proceed without an indexed gtf. That allows me to search for gene names.
For the human genome though when I press cancel nothing happens so I cannot proceed with the unindexed gtf. Would the size of the gtf be the problem? I do want to be able to look for specific genes.
Thank you
Possibly. You don't want to index the file?
I think the issue was the size. I ended up sorting and indexing the gtf file and then looking for my gene of interest by chromosome location instead of gene name.