How can I import an unindexed human gtf file in IGV?
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3 months ago
bioinfo ▴ 150

Hello,

I created an genome for IGV using a json file like shown below. I created one for both human and mouse.

{
    "id": "humanv111",
    "name": "humanv111",
    "fastaURL": "E:\\Homo_sapiens.GRCh38.111.dna.primary_assembly.fa",
    "indexURL": "E:\\Homo_sapiens.GRCh38.dna.primary_assembly.fa.fai",
    "tracks": [
      {
        "name": "Genes",
        "type": "annotation",
        "url": "E:\\Homo_sapiens.GRCh38.111.sorted.gtf",
        "indexed": false,
        "searchable": true
      }
    ]   }

For the mouse once I import it it says that it cannot find an index and I can press cancel and proceed without an indexed gtf. That allows me to search for gene names.

For the human genome though when I press cancel nothing happens so I cannot proceed with the unindexed gtf. Would the size of the gtf be the problem? I do want to be able to look for specific genes.

Thank you

igv • 267 views
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Would the size of the gtf be the problem?

Possibly. You don't want to index the file?

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I think the issue was the size. I ended up sorting and indexing the gtf file and then looking for my gene of interest by chromosome location instead of gene name.

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