How can I create an aesthetic gene neighborhood diagram from CSV data to showcase synteny?
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11 weeks ago
Rohan ▴ 20

I have a dataset in CSV format containing gene information for different species. The data includes columns for species, gene symbol, start position, stop position, and orientation. I would like to create a gene neighborhood diagram with the following specifications:

  1. Y-Axis: Species should be arranged on the Y-axis.
  2. X-Axis: Genes should be plotted on the X-axis, represented as arrows of equal size and equidistant from each other.
  3. Arrow Labels: Each arrow should have the gene symbol value written inside it.
  4. Color Coding: Arrows should be colored based on the gene symbol value.
  5. Orientation: The orientation of the arrow should reflect the orientation value (0 or 1), where 0 represents left and 1 represents right.
  6. Connecting Rectangles: Arrows with the same gene symbol across different species should be connected vertically with a rectangle shape.

Here's a sample of the data in Github Gist:

Is there a way to achieve this visualization in R? What other tools or methods should I use to create such a diagram? Any guidance or examples would be greatly appreciated.

R Plot Diagram Visualization • 524 views
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Never tried it but I just remembered this R package geneviewer mentioned on this Monday's Biostars Herald (22 july), it may be of use.

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Thank you, this could prove to be helpful.

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Please do not paste screenshots of plain text content, it is counterproductive. You can copy paste the content directly here (using the code formatting option shown below), or use a GitHub Gist if the content volume exceeds allowed length here.

code_formatting

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Thank you for your suggestion, I made the appropriate changes.

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