PCR Duplicates -- Targeted DNA NGS Panels
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10 months ago
bruceracey ▴ 10

Hi all,

I've got a simple question here. In my lab, we run various capture-based NGS panels for somatic variant detection. I'm curious what the typical PCR Duplicate rate should be for such panels. Obviously its a range, but I've mostly seen discussion of this in the context of RNA-seq. Does anyone have any experience with this, and can you share a ballpark figure for what we should be expecting?

NGS Duplicates Panel • 304 views
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