Error in GATK BaseRecalibrator
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9 months ago
Sarah • 0

I am trying to run gatk base recaliberator but keep getting this error Error showing java.lang.RuntimeException: Invalid deflate block found.

I have tried samtools quickcheck on my bam but there was no output (assuming it means no error) The BAM is 11gb. I have run many samples before using the same command but this one is really confusing me. The command I use-

singularity exec docker://broadinstitute/gatk:4.4.0.0 gatk BaseRecalibrator --intervals $intervals --interval-padding 100 -I ${ID}_P_markdup.bam -R $hg38 --known-sites $mills --known-sites $phase --known-sites $dbsnp -O ${ID}_P_recal_data.table --tmp-dir $tmp 

Please help figuring out the issue.

BaseRecalibrator gatk • 541 views
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invalid deflate block found

Looks like you may have a corrupt input file.

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