How can you determine the mutation rate of mitochondrial DNA for your (Grasshopper) species?

Question

[hashim.rana11]

I am using mitochondrial PacBio data and would like to know how to calculate the species-specific mutation rate of mitochondrial DNA for my species.? I humbly request guidance from all experts on this matter.

Please kindly correct me if I am mistaken in my approach.

Bundle of thanks in Advance!!

Best Regards,