Matrix for a Fisher's exact test

Question

[Adelya]

I want to make Fisher's exact test. I've got the number of active genes in a sample involved in EMT pathway, non-active genes, number of all the genes in a pathway and number of all the genes in a sample. Can please someone explain to me how should I place these numbers in a matrix to make Fisher's exact test?

It is 118 active, 23 non-active, 13015 in a sample and 185 in a pathway

Answer 1

[ATpoint]

The typical test for this is a hypergeometric test, not Fisher or Chi Square. See here a response from the author of clusterProfiler on exactly what you're asking: Issues with enricher() function from clusterProfiler

Answer 2

[stu.tug]

This site is quite easy to use to calculate it: https://www.langsrud.com/fisher.htm