Entering edit mode
6 weeks ago
mbyec18
•
0
Hi all, I'm a complete beginner here so please bare with me! To cut a long story short, I am analysing 16S microbiome data. I have so far completed the basics such as QC and trimming my FASTQ files, I can also convert them to FASTA and split up my FASTA files into smaller chunks for BLAST purposes.
However, the real trouble I'm having right now is getting my data to run as a QIIME2 artifact in galaxy! I cannot seem to convert my FASTQ.gz files into FASTQ.gza files so cannot use the likes of Mothur, DADA2 and QIIME2 which I need for other functions such as denoising and generating ATV's and OTU's. Any help would be appreciated, thanks!
edit: Using paired-end data generated by NGS.
Please post this over at Galaxy help forum for specific help: https://help.galaxyproject.org/