Deleted:What to do with sequences without hits after doing blast against a reference transcriptome
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12 days ago
ZuelTech • 0

Hello, I performed blast of 20 selected gene sequences against a reference transcriptome. Out of 20 sequences, 2 of them don't have hits. I adjusted the parameters into not strict (e.g., setting value from 1E-5 to 1E-3 or 1, lowering percent identity, etc.), but the result doesn't still have hits. Do you know what to do next with these 2 genes without hits in the transcriptome?

without hits transcriptome blast • 401 views
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