Entering edit mode
4 weeks ago
Analeigh
•
0
I'm working on an scRNA-seq project and fastqc keeps identifying overrepresented sequences consisting of C and T.
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT
I can’t make sense on where this could come from. Any ideas? Thanks!
Which read is it located in?
fastqc
is of limited utility with single cell data (especially 10x).CT-rich regions in introns have been reported before. At what positions in your reads does it appear? How does it affect their mapping to reference?