PacBio RNAseq Isoseq Pipeline question

Question

[evan.kransdorf]

Hello All,

I am new to PacBio RNAseq. I have a question about the pipeline - should I map the reads (using pbmm2) on the clustered BAM (i.e. has been run through isoseq cluster2).

Thank you, Evan

Answer 1

[GenoMax]

Recommended workflows for IsoSeq are here: https://isoseq.how/getting-started.html

Aligning to the genome follows clustering step.