Help with treetime ancestral MissingDataError

Question

[FJCF]

Hi everyone.

I'm trying to use treetime ancestral option but I'm getting this error:

I've checked the alignment file (run_alignment_no_resis.001.fas) and the format looks correct.

Anyone know what could be the problem?

[FJCF]

I've checked and it looks that one of the sequences is longer than the rest. Could this be what is causing the error?

[Dave Carlson]

You have not really provided enough information to say definitively, but if your alignment has sequences of different lengths, then this is definitely something you have to fix before proceeding. All sequences in a multiple sequence alignment must be the same length (when including gaps), otherwise you don't actually have a proper alignment.