Hello all,
I performed scRNA-seq analysis after overexpressing a specific gene in a particular tissue and isolating the tissue. However, upon reviewing the sequencing results, I found that the gene in the overexpression group did not show a significant increase. Moreover, transcripts of the gene were not highly captured at all.
Could this issue stem from the technical limitations of scRNA-seq? (I used the Chromium kit from 10x Genomics.)
Unfortunately, I cannot perform additional experiments, such as qPCR, as all tissue samples were used for the scRNA-seq analysis. In this case, is it still possible to derive meaningful analytical results even if the overexpression of the gene is not significantly evident in the scRNA-seq data?
Thank you for your assistance.
Best regards,
What was the method of overexpression? Is the OE gene the same species as your tissue?
Do you have any evidence the OE worked?
I think it's possible it's a detection issue, but sort of seems unlikely to me in an OE scenario