Entering edit mode
23 days ago
chentongouc
•
0
Hello, I have RNA-seq data from an F1 hybrid, as well as the genomic sequences from both the paternal and maternal parents. I want to conduct Allele-Specific Expression (ASE) analysis of F1 hybrid to determine how the expression of alleles from each parent differs. Thank you for your help!
Thank you ! I will try it!
Hello, I have other question, how can I obtain the VCF files containing the SNPs that distinguish two species? Thank you !
You usually download it somewhere. I don’t know what species you are working with, so search around.
Based on the original question you are not working with two species but an F1 hybrid. Since you have genome sequence data from the two parents you could create these VCF's yourself as long as a good reference genome for your organism is available.