Association (Protein interaction) in Intact database results what can really mean?

Question

[Bendegúz]

I'm looking for every possible interaction between proteins in Inatct database. Many results were association (not physical just "association"). The interaction detection methods were anti tag coimmunoprecipitation and anti bait coimmunoprecipitation. Confidence values were intact-miscore:0.53 and intact-miscore:0.33. In these case what could be the real interaction cell biology base meaning (compartmentation, anchoring, docking, and scaffold formation etc.) between the proteins?

Answer 1

[Mensur Dlakic]

It could mean that they are part of a large protein complex, but not necessarily involved in a binary interaction. There are many other indirect associations, such as co-expression, co-localization and the same genomic neighborhood. Most of those indicate some kind of functional links between proteins, yet they can be in the same pathway without directly interacting.

[Bendegúz]

Thanks a lot for the help and clarification. If one of the protein is a membranprotein and the other is protein has enzymefunction. Could be other special biological connection? May I have questions :) on email? :)

[Mensur Dlakic]

There are many ways in which seemingly unrelated proteins could be functionally linked. I think you will need to figure it out on your own. I don't respond to Biostars-related emails.