Hello everyone,
I am currently working on a metagenomics study where I am trying to Compare donor, responder, and non-responder gut microbiota; track differences before and after FMT, and identify statistically significant taxonomic and functional changes. However, I am a bit stuck on how to carry out the statistical analysis using R and would appreciate any pointers on how to go about this.
Background: I am working with 12 shotgun metagenome samples (4 donor, 4 responder, and 4 non-responder). The samples were preprocessed, assembled and annotated using usegalaxy. I downloaded and combined the annotated files from galaxy and imported into R for further downstream analysis. I have done the functional analysis and visualization. However, I am unable to conduct the statistical analysis as I am not sure of how to go about it.
