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5 hours ago
AlexStar
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200
In single-cell RNA-seq, there are several standard QC steps to ensure data quality, such as quantifying mitochondrial (MT) gene expression, filtering cells with a very low number of expressed genes, and examining total counts.
I'm wondering if there are equivalent, standard QC procedures for bulk RNA-seq. I know to check if deposited data is already normalized or if it consists of raw counts, but beyond that, what Exploratory Data Analysis (EDA) and QC steps are typically performed for bulk RNA-seq datasets?