**80**wrote:

Dear all, I used VCF tools to calculate Fst between two populations but now I need to calculate pairwise FST between 154 individuals within each population but I do not know how can I make this? Does anybody of you have idea how can I make this? I used this command to get Fst between the two populations:

HABIBA> vcftools --vcf adjusted_10varc30e0.vcf --weir-fst-pop TOHAMY_USDA --weir-fst-pop TOHAMY_IPK --out OUT

Thanks in advance and I am waiting your answers. Best regards, Tohamy

**11k**• written 6.2 years ago by Tohamy •

**80**