Clc Bio Blast Analysis

Question

[Edwin]

My lab just recently bought CLC Bio workbench. I am getting used to this software, but there is a simple blast analysis that I do not know why it is not giving me the results I need. I downloaded the databases from the broad Institute, I put my sequences in a fasta file, but when I retrieve my results, I can not have the description of the gene under Accession (E-value). Can anybody help me out here?

Update: I contacted both sides (CLC Bio and the Broad Institute) to ask some help. The CLC Bio people basically said that the problem is the Broad Institute database. I am waiting for Broad's response. I know it has to be something very easy, but I do not know what that is.

[Neilfws]

When you say "the description of the gene under Accession" - is that something you expect to see in the query sequence (fasta file) or the BLAST hit sequence (the BLAST database)? And if the latter - have you checked that the database was formatted so as to include that information?

[Edwin]

Thank you for your response. Yes I checked the database, and there is some information about the gene ID and its characteristics that is included in the database. I went back to check the database from the Broad Institute and the information is there. I contacted the Broad Institute to ask for help. thanks again

Answer 1

[Istvan Albert]

I think the right source of information is the provider of that data analysis package. Your purchase includes (or should include) support as well.

[Charles Warden]

Agreed: You should e-mail [e-mail]

They should reply pretty quickly - probably within 24 hours

My guess is that CLC typically works differently for a .fasta file versus a BLAST database, where you should be creating that later instead of using the former