How To Prove The Functional Implication Of Any Variation Without Performing Cell Based Assays?

Question

[nairsandhya21]

How to prove the functional implication of any variation without performing Cell based assays? Can anyone suggest any bioinformatic tools to do this?

Update: The starting material is DNA sequence. Candidate gene approach was used to find these variations.

[Pierre Lindenbaum]

"How to prove the functional implication of any variation without performing Cell based assays?" : you don't :-)

Answer 1

[Istvan Albert]

To expand on what Pierre Lindenbaum said and make this into an answered question ;-)

it is not clear what the starting information is but you always need additional sources of information to validate a hypothesis.