How to prepare GSEA Pre-Ranked input file

Question

[biorepine]

Dear Biostars, I have a file representing correlation values of certain genes (row) and transcription factors binding sites (colums). I would like to convert this file as a Pre-ranked file and submit to GSEA. Any suggestions would be appreciated.

ex:

name    tf1 tf2 tf3
gene1   0.8 0.4 0.2
gene2   -0.2    -0.6    -0.7
gene3   0.1 0.6 0.8
gene4   0.1 0   0
gene5   -0.2    -0.2    -0.2
gene6   -0.1    -0.9    -0.9
gene7   0   0   0.2

Answer 1

[Istvan Albert]

Well you will need to apply a statistic, say a t-test to come up with a p-value for each row.

See this post as well: Gsea Pre-Ranked File Format