Search of sequences of interest in the 454 data
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8.6 years ago
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Hi all,

I work with the antibody sequences (length = ~400-450 bp) and have the data from 454 platform.

From this data I want to find the sequences that are clonally related to the template or reference antibody sequence that I have. Clonally related sequences share the highly homologues CDR3 motif which is ~40-50bp long sequence present in the antibody sequence.

Schematic of antibody seq:

|-----------------------------------**********-----------------------|

***** is the CDR3 motif.

Question- I want to find the sequences from 454 data that has the same or highly homologous motif of interest in it. Which tools, programs would be useful for me. Any help/directions would be very helpful. ps- I'm beginner in bioinformatics field.

Thank you.

antibody-sequence 454 • 1.9k views
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Thanks Umer! Yes I know the sequence (I mean I have a known antibody sequence with CDR3 motif), I've never used emboss but will try. And how about BLASTing 454 reads against the 40-50 bp long CDR3 motif of this known antibody sequence, do you think its a good idea or I'll miss the hits from 454 data?

Thanks very much!

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Yeah you may do that, but use a local alignment tool by making your original 454 data as a database and your motifs as a query sequence and using something like SWIPE

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Great, thanks again! I will try the 2nd option-searching motif against 454 database. Is it possible to contact you again I just in case if I have more questions.

Best Regards, G

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Yes sure, you may contact me if you get stuck!

Best Wishes,

Umer

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