How to deal with sequencing reads that can both align to miRNA precursors and mRNA database?
0
1
Entering edit mode
10.0 years ago
dolphing ▴ 10

Hi,

I have some miRNA sequencing datasets from human, the 3' adapters were trimmed and reads length less than 18 bp were discarded. When I aligned these datasets to miRNA precursor database and mRNA database, I found that more than 80% reads can both align to these two databases.

If I want to predict novel miRNA, should I remove the reads aligned to these two databases or only remove those aligned to miRNA precursors?

Thanks in advance for your help!

RNA-Seq alignment sequencing • 2.6k views
ADD COMMENT

Login before adding your answer.

Traffic: 2900 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6