Question: How to deal with sequencing reads that can both align to miRNA precursors and mRNA database?
1
gravatar for dolphing
5.1 years ago by
dolphing10
China
dolphing10 wrote:

Hi,

I have some miRNA sequencing datasets from human, the 3' adapters were trimmed and reads length less than 18 bp were discarded. When I aligned these datasets to miRNA precursor database and mRNA database, I found that more than 80% reads can both align to these two databases.

If I want to predict novel miRNA, should I remove the reads aligned to these two databases or only remove those aligned to miRNA precursors?

Thanks in advance for your help!

sequencing rna-seq alignment • 1.9k views
ADD COMMENTlink modified 5.1 years ago by Biostar ♦♦ 20 • written 5.1 years ago by dolphing10
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