Question: Maximum distance between paired bases in secondary structure prediction
gravatar for tomas.iesmantas
5.0 years ago by
tomas.iesmantas30 wrote:

In RNA secondary structure prediction servers like Mfold or Sfold there is an option to choose whether you want to fold it as a prokaryote or eukaryote. If one chooses to use a prokaryote option, then Sfold algorithm will use a maximum distance (of 50 bases) between paired bases. But is it really true, that for example mRNA of some prokaryote in reality there is some kind of restriction relating to maximum distance between paired bases? Is it because of circular nature of DNA the folding of mRNA will be more local, i.e. paires do not form between very distance bases?

rna • 1.1k views
ADD COMMENTlink modified 5.0 years ago by Asaf5.5k • written 5.0 years ago by tomas.iesmantas30

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ADD REPLYlink modified 5.0 years ago • written 5.0 years ago by PoGibas4.8k
gravatar for Asaf
5.0 years ago by
Asaf5.5k wrote:

From my experience the fold is relatively local and allowing for distal base-pairing won't change the predicted structure a lot. I think this has to do more with the capabilities of the algorithm than the biology. There can be such interactions but these interactions will usually involve pseudoknots which basic RNA structure prediction algorithms won't deal with anyway.

ADD COMMENTlink written 5.0 years ago by Asaf5.5k
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