Distribution of e-values in Best Reciprocal Blast Hits for Ortholog Mining
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7.5 years ago
pld 5.0k

Does anyone have an idea of how the distribution of expect values will look when using reciprocal blast to detect orthologs?

blast ortholog brbh rbh • 1.6k views
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Entering edit mode
7.5 years ago
SES 8.5k

E-values are dependent on the length of the database, so they are not really comparable between analyses. Other reasons you can't directly compare the distribution of e-values is because the duplication history and divergence level between the species being compared might vary between analyses. I would look at the distribution of Ks values, that is commonly used and it will give you something easier to interpret.

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