Question: DNA sequence in bacterial genomes
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gravatar for raminkm2000
6.1 years ago by
France
raminkm20000 wrote:

I am newbie in Bioinformatics. I have downloaded all the bacterial genomes (all.fna) from
ftp://ftp.ncbi.nlm.nih.gov/genomes/Bacteria/

I have some questions about the genomes and I will be appreciated if you help me to find the appropriate answer.

Are the bacterial genomes for a single strand or double strand?

In case of single strand how can I obtain the inverse strand. Should I start to read from the last letter in the genome and change the letters A --> T, C-->G, G-->C and T-->A, or its not a matter if I start from the first vs last letter in the genome?

genome • 1.3k views
ADD COMMENTlink modified 6.1 years ago by Devon Ryan95k • written 6.1 years ago by raminkm20000
1
gravatar for Devon Ryan
6.1 years ago by
Devon Ryan95k
Freiburg, Germany
Devon Ryan95k wrote:

The opposite strand is the reverse complement of the forward (DNA is always written 5' to 3'). So the opposite of ATGC is GCAT.

ADD COMMENTlink written 6.1 years ago by Devon Ryan95k
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