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Answer: Should I scale all genes in single cell Seurat?
Answer: Should I scale all genes in single cell Seurat?
Answer: Should I scale all genes in single cell Seurat?
Answer: Problematic fastq files...How can we trust them?
Comment: How can I adjust Y-axis scale when making relative abundance box plot ?
Comment: How can I adjust Y-axis scale when making relative abundance box plot ?
Comment: How can I adjust Y-axis scale when making relative abundance box plot ?
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Recent Replies
Comment: scRNA-seq: Consistent low number of cells and low fraction reads across the samp
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I wonder if using cell hashing, which would allow pooling of cells from multiple samples before droplet formation, could improve things her…
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And the hg38 version can be found here: https://hgdownload.soe.ucsc.edu/goldenPath/hg38/database/cytoBand.txt.gz And for convenience, upda…
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You added a position dodge on your boxplot but not on your points.
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Please understand that biostars is not a helpdesk for trivial ggplot questions. Google it and find help on previous StackExchange sites, or…
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Hello, I am reviewing some old scripts in which I used clumpify.sh (BBMap 38.90) with the following parameters: ./clumpify.sh in1=$dir_c…
Comment: Problematic fastq files...How can we trust them?
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Istvan Albert
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perhaps ask her about the origin of the data, how was the data postprocessed FASTQ data is a bit more resilient than other types of data a…
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If you have an organism that lacks the GO to gene mapping then you have to find a way to assign these terms to your genes. Basically your …
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you have not mentioned what kind of resources you have, how much data you have to process and what the expected genome/transcriptome sizes …
Comment: Problematic fastq files...How can we trust them?
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ATpoint
78k
Then tell her to bring proper data. In the lab you also don't use buffers with mold growing in it, and in bioinformatics we don't start wit…
Comment: Problematic fastq files...How can we trust them?
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blackadder
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Its not my data. An external researcher is visiting and she wanted from us to do some bioinformatics... The samples were not sequenced by u…
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Oh, I understand. Thank you so much for your help! The truth is that there is so much information, so many packages, libraries, tutorials,…
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Just to clarify: Many ML models are trained and executed using dedicated software libraries for machine learning and artificial intellige…
Answer: Problematic fastq files...How can we trust them?
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ATpoint
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> I assume that maybe all these are fixable with a script but my question is, can we trust these files? Is it worth spending time and effor…
Comment: Tools or R / Python packages to perfom ML models
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sil_bioinfo
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My main idea is to be able to classify patients with active TB, LTBI and healthy patients as well as possible. The problem is that within t…
Comment: scRNA-seq: Consistent low number of cells and low fraction reads across the samp
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rpolicastro
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They could have saved themselves a ton of money by sequencing this on a miseq.
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