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31k
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34 follow
Forum: List of cloud genomics companies
cloud-genomics
updated 14 days ago by 22k • written 10.4 years ago by ★ 3.6k
12
votes
7
replies
7.0k
views
Forum: Differences between SMART-seq2, SMART-seq3, and 10x
cell smartseq UMIs single 10x
updated 29 days ago by ★ 5.8k • written 2.6 years ago by ▴ 70
12
votes
14
replies
2.4k
views
8 follow
Forum: Recovering bam files after unknown deletion in the storage
photorec bam sequencing
written 4.9 years ago by ▴ 70
16
votes
10
replies
1.0k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 25 days ago by 5.3k • written 25 days ago by ★ 2.4k
7
votes
7
replies
1.0k
views
Forum: Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career advice
updated 7 days ago by ★ 2.5k • written 8 days ago by • 0
5
votes
2
replies
374
views
Forum: Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 29 days ago by 54k • written 29 days ago by • 0
1
vote
2
replies
327
views
Forum: VCF bulk handling
bcftools SnpEff WGS VEP VCF
6 days ago by Javier • 0
4
votes
2
replies
270
views
Forum: Bioinformatics: Where do I start
Sequencing
updated 6 days ago by 43k • written 7 days ago by ▴ 10
1
vote
1
reply
211
views
Forum: Can I use this community for software development?
software-development
updated 28 days ago by 43k • written 29 days ago by • 0
0
votes
0
replies
71
views
Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
10 hours ago by yxwucq • 0
10 results • Page 1 of 1
Recent Votes
Comment: Failed kmer content
Using vg gamsort with naive sorting algorithm
Answer: Using vg gamsort with naive sorting algorithm
Interested in Spatial Omics?
Interested in Spatial Omics?
Comment: Downsampling fastq file
Answer: Running blastp with BLAST+ 2.15.0 against custom database; need to identify hits
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Recent Replies
Comment: Subset Seurat object from Xenium spatial data by bk11 ★ 2.3k
For subsetting a sample, you can do something like this- Idents(seuratObj) <- "orig.ident" subj1 <- c("subject_1") subj1_seur…
Comment: Redirection of Duplicate PMIDs by LauferVA 4.2k
Ok! I have a recommendation. I'll submit it as an answer, pending your feedback. vincent
Comment: DYH17 Structure Prediction by anasjamshed ▴ 120
In modeller we need to use templates as well.so 63% is acceptable
Comment: DYH17 Structure Prediction by Ram 43k
Mensur said > The latter option assumes you know how to use Modeller Do you know how to use Modeller? I vaguely recall there being a mini…
Comment: Should I remove unlocalized scaffols in reference genome before alignment? by GenoMax 141k
OP is working with long reads so the chances of reads aligning to unlocalized scaffolds are relatively small. Doing some testing may be suf…
Comment: What is the real meaning of relative enrichment/peak height of ChIP-seq tracks? by ATpoint 81k
I personally think that comparing the same peak between conditions is meaningful and comparing peaks within the same sample is not.
Comment: Should I remove unlocalized scaffols in reference genome before alignment? by ATpoint 81k
These regions are present in the genome and removing them might incorrectly redirect alignments to known genes. I don't see why you would r…
Comment: Should I remove unlocalized scaffols in reference genome before alignment? by njornet ▴ 20
Ok thank you! Do you know if there is an easy way of doing so with ncbi-genome-download or I need to use another tool?
Comment: Using vg gamsort with naive sorting algorithm by Pierre Lindenbaum 161k
> Don't forget to follow up on your threads. If an answer was helpful, you should upvote it; if the answer resolved your question, you shou…
Comment: Redirection of Duplicate PMIDs by dominickd • 0
The scope is all publications related to grants from an NIH award program. There are currently ~120k publications in total (about a few hun…
Answer: Using vg gamsort with naive sorting algorithm by anovak ▴ 120
The `-d` sorting mode does the entire sort in memory, so it will only work if you have enough memory to load your whole GAM file. To fin…
Comment: Redirection of Duplicate PMIDs by dominickd • 0
I have a list of PMIDs for publications linked to a list of grants, exported from NIH RePORTER.
Comment: Plot DNA methylation level of DMR on the basis of certain genes by Pierre Lindenbaum 161k
> Don't forget to follow up on your threads. If an answer was helpful, you should upvote it; if the answer resolved your question, you shou…
Comment: Should I remove unlocalized scaffols in reference genome before alignment? by GenoMax 141k
Yes you can remove those if you are only interested in annotated genes.
Comment: Help for coding: Trinity for differential gene expression studies by mchour • 0
[This could help][1] [1]: https://github.com/trinityrnaseq/RNASeq_Trinity_Tuxedo_Workshop/wiki/Trinity-De-novo-Transcriptome-Assembly-W…
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