Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : today
all time
today
this week
this month
this year
1 result • Page
1 of 1
Sort: Rank
Rank
Views
Votes
Replies
0
votes
0
replies
16
views
Forum:
How to deeply sequence long inserts
NGS
phage
read
display
Long
1 hour ago by
Ryan
• 0
1 result • Page
1 of 1
Recent Votes
Comment: Identify clusters of populations based on gene expression
Answer: How can I map coordinates between two strains of yeast?
Comment: Construction of single sequence assembly out of contigs
Answer: featureCounts results: low rate of 'Successfully assigned alignments'
The Biostar Herald for Monday, November 27, 2023
Comment: use bioservices.UniProt (python) to map uniprot accession to [multiple] ensembl
Answer: Best practices for unstranded sequences in featureCounts
Recent Locations •
All
United States,
just now
Belgium,
1 minute ago
Canada,
1 minute ago
Spain,
2 minutes ago
University of Vigo,
3 minutes ago
United States,
4 minutes ago
United Kingdom,
5 minutes ago
Recent Awards •
All
Popular Question
to
Christian
▴ 30
Popular Question
to
sehriban.buyukkilic
• 0
Popular Question
to
joe_genome
▴ 40
Popular Question
to
biohacker_tobe
▴ 80
Popular Question
to
pixie@bioinfo
★ 1.5k
Popular Question
to
doggie
• 0
Popular Question
to
Hamtaro
▴ 10
Recent Replies
Comment: How can I map coordinates between two strains of yeast?
by
dthorbur
▴ 840
I think that is an alternative to a synteny analysis, but I only know liftover in terms of annotations. OP asks for "coordinates between th…
Comment: Bowtie mapping for single_end read
by
ATpoint
78k
I am sceptical any tweaking of parameters will make a notable difference. Parameters in bowtie are very good at default, and given it's ChI…
Comment: How can I map coordinates between two strains of yeast?
by
cmdcolin
★ 3.4k
would something like liftover not be recommended? honest question
Comment: Best practices for differential expression analysis with low-yield Nanopore/ONT
by
dthorbur
▴ 840
That's good. But I still suspect this data might be really difficult to analyse. I have a handful of suggestions I would do to assess wheth…
Comment: Identify clusters of populations based on gene expression
by
dthorbur
▴ 840
One gene might not be enough data to "cluster" based on expression. Unless I'm missing something, that would just be breaking a single cont…
Comment: Best practices for differential expression analysis with low-yield Nanopore/ONT
by
tw_140
• 0
Yes, I performed ribosome depletion using an RNAse H based method.
Comment: Circular genome browser
by
cmdcolin
★ 3.4k
that's great to hear. one "proxy" for interactive is looking at the JS based tools, many are web based and interactive as a result. I have …
Comment: R ComplexHeatmap - Dividing Column Annotation into Distinct Y-Axis Scales
by
TC_Chang
▴ 10
Hi jv, Thank you for your reply. Basically, I'm trying to generate the figure shown below with two different y-axis scales. Any suggestio…
Comment: Best practices for differential expression analysis with low-yield Nanopore/ONT
by
dthorbur
▴ 840
I don't have a suggestion for low yield DE analyses, but I am curious about the samples. Did you conduct any ribo-depletion steps prior to …
Answer: How can I map coordinates between two strains of yeast?
by
dthorbur
▴ 840
It sounds like you are looking for synteny between the two genomes. I'm assuming they are assembled, but that may not be the case. If so, […
Comment: Identify clusters of populations based on gene expression
by
Nitin Narwade
★ 1.5k
Why don't you just plot it on your UMAP If it is specific to one cluster/sub-population you should get a relatively high expression in that…
Answer: The difference blastn output when using subject and db options
by
GenoMax
136k
Default `task` option is `megablast` for `blastn` which looks for `highly similar sequences` compared to `blastn` which is looking for `som…
Comment: Discrepancy in total number of bases in trimmed read1 and read2 files after BBDu
by
GenoMax
136k
https://www.biostars.org/u/14684/ may comment on this but it may be the order of operations that is causing this. After the `tpe` is applie…
Comment: Construction of single sequence assembly out of contigs
by
colindaven
5.7k
Inspector (only for long reads) and Merqury for short reads are also pretty good methods for doing a QC of your assembly.
Comment: featureCounts results: low rate of 'Successfully assigned alignments'
by
GenoMax
136k
> why I have soo many multimapping reads? That is a characteristic of the libraries. Not much you can do at this point in process. Just ha…
Traffic: 2504 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6