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460 results • Page 2 of 10
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19
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15
replies
2.0k
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6 follow
Forum: Cross out line in Biostars
meta
updated 11 months ago by 43k • written 5.9 years ago by 5.3k
10
votes
15
replies
2.1k
views
Forum: How should reference genome fasta files be distributed by UCSC?
reference-genome fasta freeze ucsc-genome-browser
updated 10 months ago by 22k • written 14 months ago by ★ 1.6k
23
votes
15
replies
5.1k
views
6 follow
Forum: On the usage of Golang
assembly next-gen alignment
updated 11 months ago by 43k • written 5.5 years ago by ▴ 80
22
votes
15
replies
8.8k
views
11 follow
Forum: RNASeq user experience with Novogene?
RNA-Seq
updated 11 months ago by 43k • written 5.8 years ago by ▴ 80
21
votes
15
replies
2.1k
views
9 follow
Forum: Working abroad without a PhD
career
updated 10 months ago by 43k • written 4.6 years ago by ★ 1.4k
6
votes
15
replies
1.7k
views
Forum: PhD Bioinformatics on GWAS
SNP GWAS genome PHD
updated 10 months ago by 43k • written 4.2 years ago by ▴ 50
57
votes
15
replies
6.2k
views
9 follow
Forum: Good practices while dealing with very large datasets
Big-Data Programming
updated 11 months ago by 43k • written 7.0 years ago by ★ 1.2k
12
votes
14
replies
2.4k
views
8 follow
Forum: Recovering bam files after unknown deletion in the storage
photorec bam sequencing
written 4.9 years ago by ▴ 70
17
votes
14
replies
3.3k
views
Forum: Asking for feedback on a Python library for computing alignments
alignment Python
updated 10 months ago by ▴ 220 • written 2.3 years ago by ▴ 60
19
votes
14
replies
2.1k
views
Forum: What is the amount of sequencing data produced annually?
data research sequencing
updated 7 months ago by 22k • written 7 months ago by ▴ 70
3
votes
14
replies
2.1k
views
6 follow
Forum: Search for gene variants
next-gen
updated 11 months ago by 43k • written 5.7 years ago by 8.3k
5
votes
14
replies
2.0k
views
Forum: immediate help needed for genome sequence analysis of bacteria
genome miseq webinar paired-end
updated 11 months ago by 43k • written 7.1 years ago by • 0
17
votes
14
replies
1.9k
views
10 follow
Forum: What would be the trend in next few years in NGS era?
NGS sequencing
updated 11 months ago by 43k • written 5.4 years ago by ▴ 800
9
votes
14
replies
2.4k
views
Forum: MS Bioinformatics Project
assembly sequencing alignment R SNP
updated 11 months ago by 43k • written 6.1 years ago by ▴ 10
73
votes
14
replies
4.2k
views
12 follow
Forum: Things you wish you knew when entering the bioinformatics field
pitfalls advice
updated 10 months ago by 43k • written 3.6 years ago by ★ 16k
11
votes
13
replies
4.5k
views
6 follow
Forum: NGS Data storage solutions for small organisations or big labs
big-data HTS storage NGS metagenomics
updated 10 months ago by 43k • written 4.9 years ago by ▴ 830
1
vote
13
replies
2.8k
views
Forum: Bioinformatics computer upgrade help
computer
updated 10 months ago by 43k • written 3.8 years ago by ▴ 50
30
votes
13
replies
3.7k
views
11 follow
Forum: what is your daily must see bioinformatics web site?
learn technology
updated 10 months ago by 43k • written 9.6 years ago by 9.7k
4
votes
13
replies
1.7k
views
Forum: Looking for Participants: Meenta NovaSeq RunShare Beta
RunShare next-gen NovaSeq
updated 10 months ago by 43k • written 4.8 years ago by ▴ 10
15
votes
13
replies
4.1k
views
8 follow
Forum: Does a PhD or work experience set you up better to do bioinformatics work in industry?
advice industry phd
updated 3 months ago by 3.9k • written 17 months ago by ▴ 30
7
votes
13
replies
1.3k
views
Forum: How do you validate and verify your pipeline's software updates?
Validation Verification Quality-Assurance
updated 6 months ago by 43k • written 6 months ago by ▴ 10
18
votes
12
replies
2.5k
views
6 follow
Forum: why GATK makes things more complicated ?
genomics
updated 11 months ago by 43k • written 5.2 years ago by ▴ 280
22
votes
12
replies
1.8k
views
6 follow
Forum: What were the most impactful Bioinformatics papers published this year
papers publications
updated 10 months ago by 43k • written 4.4 years ago by ▴ 520
12
votes
12
replies
3.0k
views
Forum: Programming interview via Skype
programming-test interview
updated 11 months ago by 43k • written 5.8 years ago by ▴ 50
4
votes
12
replies
1.5k
views
Forum: publicly available data
arrayexpress GEO SRA
updated 10 months ago by 43k • written 4.5 years ago by ▴ 110
21
votes
12
replies
3.5k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
3 months ago by Ram 43k
12
votes
12
replies
1.4k
views
7 follow
Forum: Who qualify as a world class bioinformatician as seen in job ads
world-class bioinformatician qualification
updated 10 months ago by 43k • written 4.0 years ago by • 0
26
votes
12
replies
7.5k
views
6 follow
Forum: Mapping God Found ‘Scientifically Dishonest’ By Anonymous Peer Reviewers
ngs
updated 11 months ago by ▴ 10 • written 10.5 years ago by ▴ 50
23
votes
12
replies
1.9k
views
6 follow
Forum: Whole-Exome Analysis on a Mainstream Desktop PC
NGS whole-exome
updated 10 months ago by 43k • written 3.8 years ago by 11k
31
votes
12
replies
933
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
7 weeks ago by karlaarz ▴ 90
13
votes
11
replies
4.7k
views
Forum: Anyone using Ubuntu on Windows (WSL)?
ubuntu wsl
updated 11 months ago by 43k • written 5.8 years ago by ▴ 250
18
votes
11
replies
2.4k
views
8 follow
Forum: Create a File format to store Variant
hgvs json variant
updated 11 months ago by 43k • written 5.8 years ago by ★ 2.4k
17
votes
11
replies
973
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by sil_bioinfo ▴ 40
21
votes
11
replies
2.0k
views
Forum: Most important International Big Data Projects: Genetics and Epigenetics
big-data
updated 11 months ago by 43k • written 5.7 years ago by ★ 9.4k
22
votes
11
replies
3.3k
views
Forum: ExpressionSet in the age of tidyverse
expressionset R bioconductor
updated 11 months ago by 43k • written 6.0 years ago by 13k
10
votes
11
replies
4.2k
views
7 follow
Forum: What made you interested in Bioinformatics?
career
updated 11 months ago by 43k • written 6.4 years ago by ▴ 990
14
votes
11
replies
6.7k
views
7 follow
Forum: Do you need a deduplication tool for FASTQ data in fastp?
deduplication fastp
updated 10 months ago by 43k • written 4.9 years ago by ★ 2.5k
23
votes
11
replies
2.0k
views
9 follow
Forum: file and directory management best practices
file-management
updated 10 months ago by 43k • written 3.7 years ago by ▴ 140
21
votes
11
replies
2.5k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 5 months ago by 22k • written 13 months ago by ▴ 150
8
votes
11
replies
1.7k
views
Forum: What makes a good bionformatics prediction service from the point of user experience?
server prediction-service
updated 11 months ago by 43k • written 6.5 years ago by ▴ 40
9
votes
11
replies
3.2k
views
7 follow
Forum: Fields with biggest demand for bioinformatics?
career
updated 11 months ago by 43k • written 5.3 years ago by ▴ 200
15
votes
11
replies
4.7k
views
9 follow
Forum: Methodological problems are extremely common for enrichment analysis - beware the pitfalls before you publish
GSEA DAVID enrichment-analysis -geneontology
updated 11 months ago by 43k • written 2.1 years ago by ★ 1.9k
21
votes
11
replies
1.9k
views
8 follow
Forum: what I want to do after the PhD?
PhD
updated 11 months ago by 43k • written 7.1 years ago by ★ 4.3k
25
votes
11
replies
3.7k
views
6 follow
Forum: Site Use Guidelines
meta biostars Guidelines
updated 7 hours ago by 43k • written 14.6 years ago by 100k
16
votes
10
replies
1.0k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 25 days ago by 5.3k • written 26 days ago by ★ 2.4k
9
votes
10
replies
1.3k
views
6 follow
Forum: What's more clear, loops or functions?
R
updated 11 months ago by 43k • written 5.5 years ago by ▴ 110
15
votes
10
replies
2.3k
views
6 follow
Forum: help identifying bioinformatics operations tools
database next-gen operations genomics
updated 11 months ago by 43k • written 5.4 years ago by ▴ 80
2
votes
10
replies
1.2k
views
Forum: AddHealth Omics Data Source
AddHealthOmics
updated 11 months ago by 43k • written 5.4 years ago by • 0
9
votes
10
replies
1.7k
views
Forum: Brainstorming: applying bioinformatics NOT to public field. Ideas and perspectives
applied-sciences data-analysis
updated 11 months ago by 43k • written 6.1 years ago by ★ 1.3k
26
votes
10
replies
4.4k
views
6 follow
Forum: Why not merging?
fastq sequencing
updated 11 months ago by 43k • written 6.8 years ago by 16k
460 results • Page 2 of 10
Recent Votes
Comment: What is the real meaning of relative enrichment/peak height of ChIP-seq tracks?
Comment: TCGA2STAT Error: Firehose connection
Answer: HaplotypeCaller - only SNPs
Comment: Bwa sampe - BGI
Comment: Redirection of Duplicate PMIDs
Answer: Redirection of Duplicate PMIDs
A: Should I Remove All Positions Containing A Gap In A Multiple Alignment Prior To
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Recent Replies
Comment: What is the real meaning of relative enrichment/peak height of ChIP-seq tracks? by HyperEvo • 0
Ok, thank you so much~
Comment: GTF file for Rhinolophus sinicus by GenoMax 141k
I was able to get the GTF file. I tried the fasta genome and it seemed to work but I did not complete the download. If you choose to ignore…
Comment: some error in building kraken2 database by Art1ess • 0
I have 2 files output, no error logs... the .kreport file: 100.00 160136511 160136511 U 0 unclassified head .res…
Comment: TCGA2STAT Error: Firehose connection by LauferVA 4.2k
agree. from a data-centric (rather than software centric) standpoint, it shouldn't be hard to get the TCGA data you are looking for from o…
Comment: Redirection of Duplicate PMIDs by LauferVA 4.2k
yep. in this case id definitely start with the grant numbers themselves as others have indicated. i did not recommend this before due to un…
Comment: GTF file for Rhinolophus sinicus by atowns21 • 0
This download doesn't seem to work for me. There is a message on the website saying "Warning: contaminated. Status: RefSeq GCF_001888835.1 …
Answer: HaplotypeCaller - only SNPs by Pierre Lindenbaum 161k
Filter the vcf dowstream after haplotypecaller using bcftools or gatk variantfiltration
Answer: Gene set enrichment analysis differences between 2020 and 2024 by geneontologyhelp ▴ 360
We have releases about monthly in order to keep our data accurate and reflect current understanding. In 4 years, there have been about 40 r…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie by GenoMax 141k
Let us know when you find out. Would be a useful thing to know what the data looks like.
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie by atowns21 • 0
Yes, that is a very good idea. Thanks for the help!
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie by GenoMax 141k
Only thing would be to try them out. See if you can detect them in the data you have. You could also simply look for unique representativ…
Comment: How to calculate identity percentage between proteins contained in a FASTA file? by GenoMax 141k
`clustal omega` can calculate distance matrix between two proteins (makes sense if your proteins are of similar size). You could try that: …
Comment: Should I use unpaired reads from trimmomatic by swbarnes2 14k
If the unpaired are _2, files, the _1 must exist somewhere. But the simple answer is probably going to be to just use all the _2 only. Yo…
Comment: Is it Acceptable to Have Uniform Quality Scores in a FASTQ File? by GenoMax 141k
> The needless granularity (fake precision) was obvious from the start You are assuming that all data generated is always of great quality…
Answer: Blasting two protein sequences vs two nucleotide sequences by Istvan Albert 100k
There are various versions of blast, that search in translated spaces, see blastx and tblastn https://blast.ncbi.nlm.nih.gov/Blast.cgi …
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