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1,000 results • Page 2 of 20
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24
votes
15
replies
9.9k
views
11 follow
Forum: PhD in bioinformatics or biostatistics as a wet-lab scientist?
biology statistics PhD
updated 6 months ago by 40k • written 9.1 years ago by ▴ 70
3
votes
2
replies
9.8k
views
Forum: How To Provide Reference Sequence Dictionary To Reordersam?
picard gatk
updated 6 months ago by 40k • written 9.8 years ago by 8.2k
37
votes
19
replies
9.8k
views
10 follow
Forum: Kallisto New RNA-seq quantification method discussion
RNA-Seq
updated 5 months ago by 40k • written 7.4 years ago by ▴ 540
34
votes
12
replies
9.8k
views
9 follow
Forum: Is going back to the wet lab worth it?
careerwet-lab
updated 5 months ago by 40k • written 6.7 years ago by ▴ 790
10
votes
6
replies
9.7k
views
Forum: Is it worth getting a Masters in Bioinformatics, financially?
career
updated 6 months ago by 40k • written 9.3 years ago by ▴ 20
0
votes
2
replies
9.5k
views
Forum: Secondary Structure Prediction software RNA/DNA
dna secondary-structure software rna
updated 6 months ago by 40k • written 8.9 years ago by ★ 3.6k
50
votes
8
replies
9.2k
views
6 follow
Forum: Encode Commentary From Dan Graur
encode
updated 7 months ago by 40k • written 10.6 years ago by 16k
5
votes
4
replies
9.2k
views
Forum: List of unresolved problems in Computational Biology / Bioinformatics?
computational-biology problems
updated 6 months ago by 40k • written 8.7 years ago by ★ 1.3k
86
votes
29
replies
9.1k
views
9 follow
Forum: What is the future of EMBL-EBI (and European bioinformatics in general) in case of a 'Brexit'?
EU brexit EBI politics
updated 5 months ago by 40k • written 7.3 years ago by 53k
11
votes
10
replies
8.9k
views
Forum: Looking For Bioinformatics Job In Germany
bioinformatician career
updated 6 months ago by 40k • written 9.5 years ago by ▴ 520
13
votes
7
replies
8.7k
views
Forum: The struggle between fastq and fastq.gz, compressed v/s uncompressed file formats
fastq
updated 4 months ago by 40k • written 6.2 years ago by 5.8k
50
votes
4
replies
8.6k
views
Forum: How to add images to a Biostars post
how-to
7 months ago by Ram 40k
22
votes
14
replies
8.5k
views
6 follow
Forum: Too late to switch to Bioinformatics?
education career
updated 6 months ago by 40k • written 9.3 years ago by ▴ 30
79
votes
35
replies
8.5k
views
17 follow
Forum: Do bioinformaticians often break molecular biologists' hearts by being the first author?
authorship career
updated 3 months ago by • 0 • written 7.8 years ago by ▴ 650
244
votes
36
replies
8.5k
views
32 follow
Forum: Good Habit for Bioinformatics Analyst or Scientist
Analyst Scientist
updated 5 months ago by 40k • written 7.4 years ago by ★ 9.3k
21
votes
17
replies
8.4k
views
7 follow
Forum: In YOUR personal opinion, who are the top 5 bioinformatics researchers in NGS in 21st century?
next-gen
updated 5 months ago by 40k • written 6.7 years ago by ▴ 10
12
votes
4
replies
8.4k
views
Forum: Bioinformatics/Biotech Firms In India
career
updated 7 months ago by 40k • written 11.4 years ago by ▴ 100
27
votes
21
replies
8.3k
views
9 follow
Forum: Better for my career: Degree in computer science or Phd in bioinformatics?
computer-science phd
updated 5 months ago by 40k • written 7.0 years ago by ★ 1.6k
99
votes
21
replies
8.3k
views
14 follow
Forum: How Has Bioinformatics Improved Over Time?
bioinformatics
updated 7 months ago by 40k • written 10.7 years ago by ▴ 370
9
votes
4
replies
8.3k
views
Forum: Biofinysics - How does bowtie2 assign MAPQ scores?
alignment bowtie2
updated 6 months ago by 40k • written 9.1 years ago by 7.9k
10
votes
3
replies
8.2k
views
Forum: Got Multiple Cpus? Activate Them With Gnu Parallel
parallel command-line ngs
updated 6 months ago by 40k • written 9.8 years ago by ★ 4.3k
9
votes
19
replies
8.2k
views
Forum: Careful!! Velvet Generates Different Assemblies From The Same Input Data And Same Input Parameters With Openmp Enabled.
velvet genome
updated 6 months ago by 40k • written 9.8 years ago by ▴ 650
13
votes
5
replies
8.1k
views
Forum: Funding Opportunities In Bioinformatics
grant
updated 7 months ago by 40k • written 10.6 years ago by 20k
46
votes
9
replies
8.1k
views
10 follow
Forum: Bioinformatics For Clinicians - What To Teach Them?
education
updated 6 months ago by 40k • written 9.8 years ago by ▴ 140
30
votes
19
replies
8.0k
views
6 follow
Forum: Programming language use distribution from recent programs / articles
programming-language programs
updated 4 months ago by 40k • written 6.4 years ago by 11k
64
votes
8
replies
7.9k
views
7 follow
Forum: Isaac Asimov Accurately Predicts Woes Of Bioinformatician
bioinformatician
updated 6 months ago by 40k • written 10.4 years ago by 20k
4
votes
6
replies
7.9k
views
Forum: TCGA: How to tell if the data is exome or WGS?
wgs mutations tcga exome
updated 11 months ago by 40k • written 8.1 years ago by ▴ 110
26
votes
0
replies
7.9k
views
Forum: Heng Li of BWA and Samtools uses this
uses-this
updated 6 months ago by 40k • written 9.0 years ago by 98k
145
votes
56
replies
7.9k
views
17 follow
Forum: Suggesting The Removal Of Post Closing
biostars
updated 7 months ago by 40k • written 10.5 years ago by 98k
19
votes
9
replies
7.9k
views
6 follow
Forum: How to support bioinformatics tools via Biostars: prefill and watch tags, reply via email
biostars
updated 7 months ago by 40k • written 9.4 years ago by 98k
47
votes
37
replies
7.8k
views
12 follow
Forum: The Organisation Of Really Contrived Acronyms (Orca)
acronyms
updated 6 months ago by 40k • written 10.1 years ago by 2.3k
3
votes
5
replies
7.8k
views
Forum: PopGenome nucleotide diversity
next-gen SNP R gene
updated 5 months ago by 40k • written 7.6 years ago by ▴ 10
49
votes
36
replies
7.8k
views
11 follow
Forum: New features and bug fixes for Biostar to work on during Google Summer of Code
meta biostars gsoc
updated 7 months ago by 40k • written 9.4 years ago by ▴ 110
28
votes
14
replies
7.7k
views
8 follow
Forum: Should I work first in a Bioinformatics company or get a Ph. D.?
Biochemistry PhD career
updated 5 months ago by 40k • written 7.4 years ago by ▴ 20
22
votes
15
replies
7.6k
views
11 follow
Forum: RNASeq user experience with Novogene?
RNA-Seq
updated 4 months ago by 40k • written 5.2 years ago by ▴ 80
68
votes
48
replies
7.6k
views
16 follow
Forum: Stranger Things: unexpected limitations of popular tools
samtools hisat2
updated 4 months ago by 40k • written 6.2 years ago by 98k
60
votes
28
replies
7.6k
views
27 follow
Forum: Jim Robinson of the Integrative Genomics Viewer (IGV) uses this
uses-this
updated 6 months ago by 40k • written 9.0 years ago by 98k
5
votes
4
replies
7.5k
views
Forum: Masters In Bioinformatics Vs Masters In Information Technology (Computer Science)
career
updated 6 months ago by 40k • written 9.5 years ago by • 0
116
votes
35
replies
7.5k
views
18 follow
Forum: Is Biostar Killing The Bioinformatics Core?
core biostars
updated 7 months ago by 40k • written 10.5 years ago by 21k
34
votes
19
replies
7.4k
views
12 follow
Forum: Programming Language In Bioinformatics
perl python
updated 6 months ago by 40k • written 10.4 years ago by ▴ 260
5
votes
6
replies
7.4k
views
Forum: Msc Bioinformatics Project Ideas
project
updated 7 months ago by 40k • written 11.4 years ago by ▴ 50
8
votes
12
replies
7.4k
views
9 follow
Forum: Mac Osx Confusing Vcf With Vcard
vcf
updated 7 months ago by 40k • written 10.5 years ago by ▴ 80
42
votes
15
replies
7.4k
views
8 follow
Forum: Directions In Bioinformatics And Web-Based Data Visualization: Revisiting Javascript?
data-visualization python r
updated 7 months ago by 40k • written 10.9 years ago by 5.7k
29
votes
4
replies
7.2k
views
Forum: Update to tbb (2021.1.1, conda-forge) breaks bowtie2 (bioconda)
software-error bowtie2 bioconda conda tbb
updated 6 months ago by 40k • written 2.5 years ago by ▴ 240
16
votes
6
replies
7.2k
views
7 follow
Forum: What computer skills should I put on my resume?
resume genome
updated 4 months ago by 40k • written 5.8 years ago by ▴ 30
9
votes
1
reply
7.1k
views
Forum: Master in statistics or bioinformatics
masters career statistics
updated 3 months ago by 40k • written 9.4 years ago by ▴ 50
2
votes
3
replies
7.1k
views
Forum: How To Correctly Speed Up Blast Using Num_Threads
blast blast-plus
updated 6 months ago by 40k • written 9.6 years ago by ▴ 40
26
votes
12
replies
7.1k
views
6 follow
Forum: Mapping God Found ‘Scientifically Dishonest’ By Anonymous Peer Reviewers
ngs
updated 4 months ago by ▴ 10 • written 9.9 years ago by ▴ 50
61
votes
16
replies
7.1k
views
14 follow
Forum: Rust or C++, what to learn after Go for high-performance bioinformatics tools?
library
updated 8 months ago by ★ 1.3k • written 2.9 years ago by 8.2k
68
votes
22
replies
7.0k
views
16 follow
Forum: Learning Python: list of resources
python
updated 5 months ago by 40k • written 6.9 years ago by ★ 2.8k
1,000 results • Page 2 of 20
Recent Votes
Answer: Identifying common DEGs among multiple datasets
Answer: Identifying common DEGs among multiple datasets
A: what do the headers of the rMATS output files mean?
A: what do the headers of the rMATS output files mean?
Answer: DESeq2 analysis using two featureCounts generated from different studies
Answer: DESeq2 analysis using two featureCounts generated from different studies
Answer: Assistance with Fungal Genome Annotation Using Maker and BLAST
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Recent Awards • All
Popular Question to BioinfoBee • 0
Scholar to Pierre Lindenbaum 157k
Teacher to Michael 53k
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Scholar to GenoMax 134k
Recent Replies
Comment: ATAC-seq troubleshoot - Just Noise by ATpoint 76k
Yes, definitely lots of noise and few peaks for such an experiment. Which celltype is that, and which lab protocol did you use?
Comment: DESeq2 analysis using two featureCounts generated from different studies by abedkurdi10 ▴ 190
What do you mean by gene lists?
Comment: Find potential important genes from bulk-RNA seq experiment by Michael 53k
You can use e.g. the WGCNA package for network analysis. In your case multiple samples this might mean multiple patients or a time series o…
Comment: Assistance with Fungal Genome Annotation Using Maker and BLAST by Edoardo • 0
Thank you so much for your responses. @genomax , I've downloaded both of the databases you recommended. I attempted to combine them into …
Answer: Simulate short-read RNA-seq data from long-read RNA-seq data by Mensur Dlakic ★ 24k
I think you could try doing this, but it wouldn't be appropriate. You'd be bringing long-read errors, which I believe are higher than short…
Comment: Salmon index not progressing by camillab. ▴ 130
I opened tow terminals and one was the code to build a decoy-aware transcriptome and the other was building indexes (no decoy)
Comment: Adding a control sample to bulk RNA-seq by Chris ▴ 180
Thank you for the explanation! So just want to confirm one control with replicate is totally useless even can't use as a reference?
Answer: DESeq2 analysis using two featureCounts generated from different studies by swbarnes2 13k
Comparing samples between labs is a bad idea. Better to generate gene lists from one study from one lab, and gene lists from another study…
Comment: Adding a control sample to bulk RNA-seq by swbarnes2 13k
If a sample wasn't prepared along side your treated samples, it is a different batch. If you had treated and controls in each of two diffe…
Comment: kallisto normalized TPM values without bootstraps by ATpoint 76k
You don't need sleuth. Typically these deconvolutions, or most downstream analysis, is done on the gene- rather than the transcript-level w…
Comment: Salmon index not progressing by ATpoint 76k
If the top $CPU column shows some values in the hundreds then it is doing something (actually when it's > 0%). So based on the screenshot i…
Comment: Salmon index not progressing by camillab. ▴ 130
Thank you. Waiting it's not gonna hurt me, when should I stop hoping and give up is the question? do you know is there a way if I can see i…
Comment: Filtering VCF to divide with equal sizes by Ram 40k
A more efficient way would be to make a 2 column CSV file, one column with the count value and the other with the chr:start-end range, then…
Answer: Filtering VCF to divide with equal sizes by avelarbio46 ▴ 30
I divided my contigs using R, but it was more complex than the program Pierre Lindenbaum made (https://jvarkit.readthedocs.io/en/latest/Vcf…
Comment: Salmon index not progressing by ATpoint 76k
Just wait until it is finished. It's seems to be running. I just checked my logs and on our HPC with four cores such as process for a decoy…
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