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460 results • Page 2 of 10
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3
votes
3
replies
900
views
Forum: Importance of Data Structures for Bioinformatics?
data-structures
updated 7 months ago by 43k • written 7 months ago by • 0
19
votes
14
replies
2.1k
views
Forum: What is the amount of sequencing data produced annually?
data research sequencing
updated 7 months ago by 22k • written 7 months ago by ▴ 70
19
votes
10
replies
1.5k
views
Forum: Pain points using commercial clouds
gpt tinybio
updated 7 months ago by ▴ 70 • written 7 months ago by ▴ 840
9
votes
6
replies
1.9k
views
6 follow
Forum: GTF files from Ensembl Releases 105 and 106 unsorted
Ensembl bug GTF
updated 7 months ago by 82k • written 17 months ago by ▴ 30
3
votes
2
replies
618
views
Forum: How to contribute to the next edition of Biostars Handbook
handbook
updated 7 months ago by 43k • written 7 months ago by ▴ 20
3
votes
6
replies
1.4k
views
6 follow
Forum: Is Bioinformatics a Professional Degree?
career degree
7 months ago by anasjamshed ▴ 120
2
votes
5
replies
946
views
6 follow
Forum: Biostar as a next generation version
meta mobile-app
updated 6 months ago by 43k • written 7 months ago by • 0
2
votes
9
replies
1.2k
views
Forum: open source projects looking for contributors
open-source
updated 7 months ago by ▴ 70 • written 7 months ago by ▴ 20
0
votes
0
replies
534
views
Forum: Recruiters: what do you expect on a junior bioinformatician?
career skills
updated 8 months ago by 43k • written 8 months ago by ▴ 20
3
votes
4
replies
1.4k
views
Forum: Discussion: Uploading terabytes of data to NCBI SRA
NCBI WGS archiving servers remote
updated 8 months ago by 141k • written 8 months ago by ▴ 130
0
votes
0
replies
564
views
Forum: I have a Master's and little experience in Bioinformatics - what would be the next best step?
career
updated 8 months ago by 43k • written 8 months ago by ▴ 10
1
vote
3
replies
768
views
Forum: Spaceranger missing spatial coordinates [Discussion]
Visium
updated 8 months ago by 141k • written 8 months ago by ▴ 550
11
votes
8
replies
1.4k
views
6 follow
Forum: How to write a review article in bioinformatics
article review
updated 7 months ago by 43k • written 8 months ago by ▴ 10
364
votes
58
replies
69k
views
44 follow
Forum: Recommend Your Favorite Bioinformatics Books
education books
updated 6 months ago by 43k • written 14.1 years ago by 100k
1
vote
2
replies
18k
views
Forum: Would you rather analyze a dataset with a strong, non-collinear batch effect, or a weak, collinear batch effect.
collinear latent batch-effect variable GLM
9 months ago by LauferVA 4.2k
0
votes
3
replies
3.8k
views
10 follow
Forum: Discussion question: Cutoff for SNP phylogeny
Phylogenetics SNP
written 16 months ago by ▴ 120
2
votes
3
replies
784
views
Forum: Purely analytic or in silico manuscripts that have significantly improved biological understanding
dry-lab
updated 9 months ago by 141k • written 9 months ago by 4.2k
0
votes
2
replies
1.3k
views
Forum: [Detailed Question] How to Normalize of Metagenomics Gene Abundances (with HMMs models)
normalization
updated 10 months ago by 10k • written 10 months ago by • 0
33
votes
23
replies
4.1k
views
8 follow
Forum: Is there a file format better suited for the era of pangenomics than the .vcf? What are its attributes?
VCF file-format genomics
updated 10 months ago by 22k • written 10 months ago by 4.2k
7
votes
8
replies
1.9k
views
Forum: Would solving many Rosalind problems show your ability for this field in job-wise?
rosalind career
10 months ago by K • 0
2
votes
3
replies
857
views
Forum: Mapping a predicted sequence to a genome
predicted map alignment sequence
updated 10 months ago by ★ 27k • written 10 months ago by • 0
0
votes
0
replies
440
views
Forum: Recommendation sought for differential exon usage of a single gene
splicing differential-exon-usage
updated 10 months ago by 43k • written 10 months ago by ▴ 10
7
votes
4
replies
1.1k
views
Forum: [Meta] Use of AI
AI Artificial-Intelligence meta ChatGPT
updated 10 months ago by 43k • written 10 months ago by 4.2k
2
votes
2
replies
594
views
Forum: Colleges
Colleges india universities
updated 10 months ago by 43k • written 10 months ago by • 0
19
votes
15
replies
2.0k
views
6 follow
Forum: Cross out line in Biostars
meta
updated 11 months ago by 43k • written 5.9 years ago by 5.3k
121
votes
33
replies
39k
views
24 follow
Forum: Where To Look For Quality Bioinformatics Short Courses And Workshops?
Courses
updated 11 months ago by ★ 2.5k • written 10.7 years ago by 11k
103
votes
42
replies
31k
views
34 follow
Forum: List of cloud genomics companies
cloud-genomics
updated 15 days ago by 22k • written 10.4 years ago by ★ 3.6k
26
votes
12
replies
7.5k
views
6 follow
Forum: Mapping God Found ‘Scientifically Dishonest’ By Anonymous Peer Reviewers
ngs
updated 11 months ago by ▴ 10 • written 10.5 years ago by ▴ 50
0
votes
2
replies
764
views
Forum: How to start in bioinformatics?
career
updated 11 months ago by 43k • written 12 months ago by • 0
4
votes
5
replies
1.4k
views
Forum: I'm looking for a mentor in Bioinformatics
mentor teaching
updated 10 weeks ago by • 0 • written 12 months ago by • 0
10
votes
15
replies
2.1k
views
Forum: How should reference genome fasta files be distributed by UCSC?
reference-genome fasta freeze ucsc-genome-browser
updated 10 months ago by 22k • written 14 months ago by ★ 1.6k
5
votes
4
replies
1.3k
views
Forum: Best BAM viewer in 2022
sam bam
updated 10 months ago by 43k • written 17 months ago by ▴ 70
1.2k
votes
206
replies
59k
views
108 follow
Forum: What Are The Most Common Stupid Mistakes In Bioinformatics?
software
updated 10 months ago by ★ 1.4k • written 13.1 years ago by 22k
15
votes
13
replies
4.1k
views
8 follow
Forum: Does a PhD or work experience set you up better to do bioinformatics work in industry?
advice industry phd
updated 3 months ago by 3.9k • written 17 months ago by ▴ 30
4
votes
5
replies
1.4k
views
6 follow
Forum: Which Bioinformatics Master's degree is better?
career masters
updated 11 months ago by 43k • written 17 months ago by • 0
3
votes
6
replies
1.1k
views
Forum: How to become a moderator and other ways of contributing to Biostars
meta biostars
updated 11 months ago by 43k • written 18 months ago by 6.2k
3
votes
2
replies
845
views
Forum: From protein-protein interaction networks to..
workflow computational knowledge limitations
updated 10 months ago by 43k • written 22 months ago by ▴ 50
15
votes
11
replies
4.7k
views
9 follow
Forum: Methodological problems are extremely common for enrichment analysis - beware the pitfalls before you publish
GSEA DAVID enrichment-analysis -geneontology
updated 11 months ago by 43k • written 2.1 years ago by ★ 1.9k
0
votes
3
replies
923
views
Forum: Project Topics on bioinformatic
Data-analysis Coding Molecular-biology
updated 11 months ago by 43k • written 2.1 years ago by • 0
3
votes
1
reply
904
views
Forum: How to download multiple .gz files from Ensembl Protists database
wget protist ensembl
updated 9 weeks ago by ★ 1.7k • written 2.2 years ago by ▴ 840
17
votes
14
replies
3.3k
views
Forum: Asking for feedback on a Python library for computing alignments
alignment Python
updated 10 months ago by ▴ 220 • written 2.3 years ago by ▴ 60
0
votes
1
reply
765
views
Forum: What are the most important papers/methods in Genomics/Bioinformatics of 2021?
publications
updated 10 months ago by 43k • written 2.3 years ago by ★ 2.5k
12
votes
7
replies
7.0k
views
Forum: Differences between SMART-seq2, SMART-seq3, and 10x
cell smartseq UMIs single 10x
updated 29 days ago by ★ 5.8k • written 2.6 years ago by ▴ 70
0
votes
0
replies
515
views
Forum: Poll about a structured description of a tool, a pipeline
survey pipeline
updated 10 months ago by 43k • written 2.7 years ago by • 0
7
votes
20
replies
2.1k
views
Forum: NGS RNA-Seq Analysis Pipeline Tophat vs STAR
STAR RNA-Seq TopHat
updated 11 months ago by 43k • written 3.3 years ago by ▴ 10
11
votes
9
replies
2.4k
views
7 follow
Forum: Feature Selection and Dimensionality Reduction in 10X scRNA-seq data
software-error pca scRNA-seq
updated 11 months ago by 43k • written 4.3 years ago by 82k
0
votes
0
replies
695
views
Forum: where to get the site mutation rate of SARS-CoV-2?
sequence sequencing
updated 11 months ago by 43k • written 3.3 years ago by ▴ 40
6
votes
5
replies
1.6k
views
Forum: Very generally speaking, what are valid criteria to decide that a sample is an outlier?
outliers
updated 11 months ago by 43k • written 3.3 years ago by ▴ 300
7
votes
4
replies
1.2k
views
Forum: Specific question on torturing the data until it confesses
ipa gene-set-enrichment statistics
updated 11 months ago by 43k • written 3.3 years ago by ▴ 300
7
votes
3
replies
1.3k
views
Forum: Is it still recommended to avoid macOS Big Sur?
mac
updated 11 months ago by 43k • written 3.3 years ago by ▴ 210
460 results • Page 2 of 10
Recent Votes
Answer: Gene set enrichment analysis differences between 2020 and 2024
Answer: Gene set enrichment analysis differences between 2020 and 2024
Interested in Spatial Omics?
Answer: Seurat merge and batch correction
Answer: Seurat merge and batch correction
Comment: Per base sequence content error in RNAseq analysis
Answer: Gene set enrichment analysis differences between 2020 and 2024
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Recent Replies
Comment: How to compare the quality of assemblies by kirillkirilenko ▴ 40
A haploid assembly is our goal. For purging we used prefix.p_ctg.gfa (assembly graph of primary contigs).
Comment: Is it Acceptable to Have Uniform Quality Scores in a FASTQ File? by Istvan Albert 100k
What I mean is that no sequencing run can be calibrated with the claimed precision. Where it could correctly distinguish between basecalls …
Comment: HaplotypeCaller - only SNPs by analyst ▴ 20
After HaplotypeCaller, you can use gatk SelectVariants to extract snps or indels from vcf file ([link to command][1]) [1]: https://gatk…
Comment: GTF file for Rhinolophus sinicus by atowns21 • 0
Ah gotcha. I guess what doesn't make sense to me is why a [paper][1] published in 2022 uses that reference genome. [1]: https://www.na…
Answer: Wilcox test using data slot (log normalized count values) of scRNA-seq between t by yxwucq • 0
The best way is to generate a pseudo-bulk aggregation then apply edgeR or DESeq2. You can also directly use wilcoxon on sc-level, since man…
Comment: How to solve this RoseTTAFold colaboratory error? by Mensur Dlakic ★ 27k
There is a dedicated Discord group that deals with everything related to ColabFold and its derivatives. Below is an invitation to join that…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie by atowns21 • 0
Hmm, okay I'm not sure what is going on, but here is what I did: 1. Downloaded a single fastq: `parallel-fastq-dump --tmpdir . --threads …
Comment: What is the real meaning of relative enrichment/peak height of ChIP-seq tracks? by HyperEvo • 0
Ok, thank you so much~
Comment: GTF file for Rhinolophus sinicus by GenoMax 141k
I was able to get the GTF file. I tried the fasta genome and it seemed to work but I did not complete the download. If you choose to ignore…
Comment: some error in building kraken2 database by Art1ess • 0
I have 2 files output, no error logs... the .kreport file: 100.00 160136511 160136511 U 0 unclassified head .res…
Comment: TCGA2STAT Error: Firehose connection by LauferVA 4.2k
agree. from a data-centric (rather than software centric) standpoint, it shouldn't be hard to get the TCGA data you are looking for from o…
Comment: Redirection of Duplicate PMIDs by LauferVA 4.2k
yep. in this case id definitely start with the grant numbers themselves as others have indicated. i did not recommend this before due to un…
Comment: GTF file for Rhinolophus sinicus by atowns21 • 0
This download doesn't seem to work for me. There is a message on the website saying "Warning: contaminated. Status: RefSeq GCF_001888835.1 …
Answer: HaplotypeCaller - only SNPs by Pierre Lindenbaum 161k
Filter the vcf dowstream after haplotypecaller using bcftools or gatk variantfiltration
Answer: Gene set enrichment analysis differences between 2020 and 2024 by geneontologyhelp ▴ 390
We have releases about monthly in order to keep our data accurate and reflect current understanding. In 4 years, there have been about 40 r…
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