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451 results • Page
1 of 10
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Views
Votes
Replies
206
votes
58
replies
38k
views
31 follow
Forum:
Will Python Take The Place Of R?
r
statistics
python
biopython
updated 25 days ago by
Ram
38k • written 9.8 years ago by
Medhat
9.5k
93
votes
50
replies
14k
views
17 follow
Forum:
Fda Sends A Warning Letter To 23Andme - Personal Genomics Service Marketing To Be Discontinued
personal-genomics
23andMe
updated 20 days ago by
Ram
38k • written 9.3 years ago by
Istvan Albert
97k
262
votes
48
replies
20k
views
37 follow
Forum:
Bioinformatics courses, workshops or training
Workshop
Training
Courses
updated 23 days ago by
Lars
▴ 970 • written 7.9 years ago by
Deepak Tanwar
★ 4.2k
105
votes
46
replies
11k
views
13 follow
Forum:
Why Does Biostar Cover Questions On Epigenetics, But Not Intelligent Design?
epigenetics
updated 22 days ago by
Ram
38k • written 9.6 years ago by
ugly.betty77
★ 1.1k
126
votes
44
replies
27k
views
16 follow
Forum:
Trimming adapter sequences - is it necessary?
NGS
adapter
RNA-Seq
updated 12 days ago by
Ram
38k • written 6.5 years ago by
Lars
▴ 970
57
votes
43
replies
14k
views
8 follow
Forum:
Best RNA-Seq aligner: A comparison of mapping tools
NGS
alignment
genome
RNA-Seq
sequence
updated 12 days ago by
Ram
38k • written 4.2 years ago by
David Langenberger
9.9k
136
votes
41
replies
20k
views
20 follow
Forum:
Are We Rude/Do We Expect Too Much From People Asking Questions On This Forum?
meta
updated 25 days ago by
Ram
38k • written 9.8 years ago by
Whetting
★ 1.6k
25
votes
39
replies
4.4k
views
8 follow
Forum:
Bioinformatics Study Group
study-group
updated 22 days ago by
Ram
38k • written 9.7 years ago by
Olivier
▴ 440
151
votes
39
replies
22k
views
20 follow
Forum:
A Farewell To Bioinformatics
bioinformatics
updated 26 days ago by
Ram
38k • written 10.2 years ago by
Martin A Hansen
3.0k
159
votes
38
replies
15k
views
22 follow
Forum:
Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
updated 26 days ago by
Ram
38k • written 10.0 years ago by
Istvan Albert
97k
47
votes
37
replies
7.3k
views
12 follow
Forum:
The Organisation Of Really Contrived Acronyms (Orca)
acronyms
updated 22 days ago by
Ram
38k • written 9.6 years ago by
Niallhaslam
2.3k
115
votes
35
replies
36k
views
20 follow
Forum:
Snakemake vs. Nextflow: strengths and weaknesses
snakemake
nextflow
Forum
updated 11 days ago by
e.r.zakiev
▴ 30 • written 5.8 years ago by
ropolocan
▴ 760
82
votes
32
replies
6.4k
views
13 follow
Forum:
Shall We Go Back To Stackexchange?
meta
updated 26 days ago by
Ram
38k • written 10.3 years ago by
14134125465346445
★ 3.6k
37
votes
30
replies
4.0k
views
7 follow
Forum:
Spam In Rss Feeds
meta
RSS
updated 26 days ago by
Ram
38k • written 10.2 years ago by
Madelaine Gogol
5.3k
60
votes
28
replies
7.2k
views
27 follow
Forum:
Jim Robinson of the Integrative Genomics Viewer (IGV) uses this
uses-this
updated 13 days ago by
Ram
38k • written 8.5 years ago by
Istvan Albert
97k
65
votes
26
replies
18k
views
13 follow
Forum:
Macosx For Bioinformatics?
linux
updated 20 days ago by
Ram
38k • written 9.3 years ago by
Eric Normandeau
11k
40
votes
24
replies
2.1k
views
13 follow
Forum:
What programming language do you use the most in your work? Which one do you like the best?
Programming
Python
R
Scripting
updated 12 days ago by
Ram
38k • written 2.1 years ago by
biotrekker
▴ 80
106
votes
23
replies
20k
views
16 follow
Forum:
How Did You Learn Good Practice In Bioinformatics/Computational Biology?
bioinformatics
updated 19 days ago by
Ram
38k • written 9.1 years ago by
epigene
▴ 570
80
votes
23
replies
4.3k
views
11 follow
Forum:
Are we facing a bioinformatician shortage?
salary
career
updated 12 days ago by
Ram
38k • written 8.4 years ago by
Christian
★ 3.0k
84
votes
22
replies
3.4k
views
12 follow
Forum:
is fortran still used in bioinformatics?
FORTRAN
R
updated 12 days ago by
Ram
38k • written 3.8 years ago by
Manoj
▴ 180
20
votes
22
replies
12k
views
11 follow
Forum:
Nextseq for analysts
basespace
casava
bcl2fastq
nextseq
updated 15 days ago by
Ram
38k • written 8.7 years ago by
Madelaine Gogol
5.3k
42
votes
21
replies
14k
views
10 follow
Forum:
A Database Of Signatures Of Selection In The 1000 Genomes Dataset
1000genomes
human
selection
updated 18 days ago by
Ram
38k • written 9.1 years ago by
Giovanni M Dall'Olio
27k
20
votes
21
replies
2.3k
views
6 follow
Forum:
Next Gen seq for Job interview
next-gen
updated 5 days ago by
Ram
38k • written 8.1 years ago by
gauravsingh.singh86
▴ 20
99
votes
21
replies
7.9k
views
14 follow
Forum:
How Has Bioinformatics Improved Over Time?
bioinformatics
updated 26 days ago by
Ram
38k • written 10.1 years ago by
Kate
▴ 370
67
votes
21
replies
5.0k
views
8 follow
Forum:
Meta: Markdown in Biostars (now enabled)
meta
7 days ago by
Ram
38k
66
votes
21
replies
5.6k
views
18 follow
Forum:
Is there anyone who was a pure biologist?
next-gen
updated 15 days ago by
Ram
38k • written 8.9 years ago by
Chong Tang
▴ 100
81
votes
21
replies
4.1k
views
16 follow
Forum:
[UPDATE] Thrown into a Bioinformatics Position
bioinformatics
updated 4 days ago by
Ram
38k • written 6.8 years ago by
Sinji
★ 3.2k
36
votes
21
replies
16k
views
9 follow
Forum:
What Is Your Favorite Conserved Protein?
protein
conservation
updated 22 days ago by
Ram
38k • written 9.6 years ago by
Mary
11k
12
votes
20
replies
2.5k
views
Forum:
Starting a career in Bioinformatics
career
updated 1 day ago by
Ram
38k • written 6.8 years ago by
rupashree.choudhury
▴ 10
50
votes
20
replies
4.9k
views
18 follow
Forum:
Explain bioinformatics to a 10 year old
bioinformatics
updated 12 days ago by
Ram
38k • written 7.0 years ago by
novice
★ 1.1k
57
votes
19
replies
26k
views
6 follow
Forum:
Attention: Bowtie2 And Multiple Hits
next-gen
bowtie
mapping
updated 27 days ago by
Ram
38k • written 10.4 years ago by
David Langenberger
9.9k
9
votes
19
replies
7.9k
views
Forum:
Careful!! Velvet Generates Different Assemblies From The Same Input Data And Same Input Parameters With Openmp Enabled.
velvet
genome
updated 20 days ago by
Ram
38k • written 9.3 years ago by
Rahul Sharma
▴ 650
34
votes
19
replies
7.1k
views
12 follow
Forum:
Programming Language In Bioinformatics
perl
python
updated 25 days ago by
Ram
38k • written 9.9 years ago by
AndreiR
▴ 260
35
votes
19
replies
3.3k
views
Forum:
Crowd Sourcing The 'Analysis' Of The 'Big Foot' Genome
bigfoot
genome
updated 26 days ago by
Ram
38k • written 10.1 years ago by
Will
4.5k
27
votes
19
replies
3.8k
views
10 follow
Forum:
Google genomics cloud platform ?
genome
cloud
updated 19 hours ago by
Ram
38k • written 7.7 years ago by
GouthamAtla
12k
72
votes
18
replies
6.7k
views
17 follow
Forum:
What Is One Book Every Bioinformatician Should Read?
books
updated 22 days ago by
Ram
38k • written 9.7 years ago by
Philipp Bayer
7.7k
33
votes
17
replies
2.7k
views
7 follow
Forum:
What bioinformatics tools/software do not get enough recognition?
software
updated 13 days ago by
Ram
38k • written 8.4 years ago by
brentp
24k
41
votes
17
replies
35k
views
15 follow
Forum:
List Of Genome Sequencing Centers/Institutes
genomics
institutes
updated 26 days ago by
Ram
38k • written 10.1 years ago by
Malachi Griffith
19k
13
votes
17
replies
4.3k
views
8 follow
Forum:
A Universal/Common Biorep System
reputation
updated 20 days ago by
Ram
38k • written 9.3 years ago by
Obi Griffith
20k
9
votes
17
replies
2.3k
views
Forum:
“Systematic pattern of excess success"
paper
statistics
updated 8 days ago by
Ram
38k • written 8.3 years ago by
mikhail.shugay
3.5k
43
votes
16
replies
4.0k
views
10 follow
Forum:
Need Advice On How To Become A Senior Bioinformatician
advice
career
updated 25 days ago by
Ram
38k • written 9.9 years ago by
calicruz206
▴ 30
20
votes
16
replies
2.8k
views
Forum:
Blacklist of questionable genbank entries
genbank
gi
stealth-virus
blacklist
updated 15 days ago by
Ram
38k • written 8.9 years ago by
Michael Dondrup
52k
22
votes
16
replies
6.4k
views
9 follow
Forum:
Large Tables In Supplementary Pdf In Journal Articles
publication
updated 22 days ago by
Ram
38k • written 9.7 years ago by
dario.garvan
▴ 490
29
votes
16
replies
2.6k
views
Forum:
Making bioinformatics help and tutorials open
ensembl
updated 13 days ago by
Ram
38k • written 8.6 years ago by
Emily
23k
42
votes
15
replies
7.0k
views
8 follow
Forum:
Directions In Bioinformatics And Web-Based Data Visualization: Revisiting Javascript?
data-visualization
python
r
updated 27 days ago by
Ram
38k • written 10.4 years ago by
Josh Herr
5.7k
24
votes
15
replies
9.3k
views
11 follow
Forum:
PhD in bioinformatics or biostatistics as a wet-lab scientist?
biology
statistics
PhD
updated 13 days ago by
Ram
38k • written 8.6 years ago by
benjamin.hebisch
▴ 70
28
votes
15
replies
4.0k
views
8 follow
Forum:
Anyone Use R For Their Bioinformatics Work?
r
updated 21 days ago by
Ram
38k • written 9.4 years ago by
arronslacey
▴ 310
16
votes
15
replies
5.7k
views
Forum:
Would You Be Interested In Having A Usesthis Like Site For The Bioinformatics Community?
hardware
software
updated 15 days ago by
Ram
38k • written 9.0 years ago by
Drio
▴ 920
8
votes
15
replies
3.5k
views
Forum:
Can Galaxy Be Used Under The Hood? How About The Galaxy Appliance?
galaxy
updated 22 days ago by
Ram
38k • written 9.7 years ago by
seidel
11k
10
votes
15
replies
819
views
Forum:
How should reference genome fasta files be distributed by UCSC?
reference-genome
fasta
ucsc-genome-browser
20 days ago by
Maximilian Haeussler
★ 1.5k
451 results • Page
1 of 10
Recent Votes
Comment: getting coding exon information from Ensembl API
C: How to install TMAP
If there is a way to link two gosling html components in two <div>s.
A: Using GATK's HaplotypeCaller or Mutect2 for somatic cancer samples with no norma
Comment: KM Plot for gene of interest (e.g. TP53) using TCGA-PAAD dataset
Comment: How can I easily remove overlapping transcripts, keeping only longest transcript
Comment: Where do find virulence gene information in a gff/gtf file?
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Recent Replies
Comment: How can I easily remove overlapping transcripts, keeping only longest transcript
by
Juke34
7.7k
Don't use `--merge_loci` in your command line this was for AGAT version <1.0.0 (and only few scripts), now this is handled via the config.y…
Comment: getting coding exon information from Ensembl API
by
Ram
38k
> Because you just answer No we don't. I think you're confusing this professional forum contributed to by volunteers with a customer suppo…
Comment: Can I use abundance from Tximport to compare the expression level of transcript
by
Ram
38k
Oh, you can just use TPM to do that. Within sample comparison is a completely different (and much easier) game.
Comment: ClusterProfiler enrichKEGG – remove organism name in plots?
by
Sian
• 0
Unfortunately yes, there does seem to be a problem with using the older version - when I came to do more analysis yesterday, the enrichKEGG…
Comment: getting coding exon information from Ensembl API
by
Sarah
▴ 30
Because you just answer when it's a stupid question or when I ask a lot of questions. This is for asking questions not for being judged by …
Comment: KM Plot for gene of interest (e.g. TP53) using TCGA-PAAD dataset
by
Manav
• 0
Thanks, tried working with the dataset and workflow. It worked.
Comment: How can I easily remove overlapping transcripts, keeping only longest transcript
by
BioinfoBee
• 0
@juke34 Thanks again. I changed the config.yaml *should overlapping loci (at CDS level) be merged in a single locus Only one gene is kept,…
Comment: Can I use abundance from Tximport to compare the expression level of transcript
by
aimanbarki
▴ 20
Thanks, I am not doing any differential. But, Can we say that 6th transcript is relatively more abundance in our sample than other transcri…
Comment: Java -xmx option does not limit memory usage in the ImputePipelinePlugin?
by
zrm22
▴ 30
It looks like the ImputePipelinePlugin for the minimap2 run does not use the numThreads Option but rather does the numThreadsOnMachine - 2 …
Comment: Can I use abundance from Tximport to compare the expression level of transcript
by
aimanbarki
▴ 20
@rob Please, Can you or people from your group answer . As I am using one of your paper as my [reference.][1] [1]: https://f1000rese…
Comment: fastq_screen error: Failed to calculate read length
by
firestar
★ 1.5k
I created the .fasta from a BAM, so I am just going to go back to the BAM and convert that to a .fastq and see if that works. But good to k…
Comment: Can I use abundance from Tximport to compare the expression level of transcript
by
Ram
38k
> I have only one condition There is no "differential" then. RNAseq is a relative measure, so comparing single-condition replicates using …
Comment: Can I use abundance from Tximport to compare the expression level of transcript
by
aimanbarki
▴ 20
[Salmon][1] is giving me TPM at transcript level. So, What I understand from that TPM is normalized for sequencing depth and Transcript l…
Answer: Does it possible to know what are the adjacent genes up to 1000bp upstream and
by
Alex Reynolds
34k
You could use `bedmap` from BEDOPS. Say you have a BED file of IS/transposable elements called `elements.bed` and a BED file of genes calle…
Comment: Java -xmx option does not limit memory usage in the ImputePipelinePlugin?
by
twrl8
• 0
Ahh thank you! I thought the xmx parameter would be passed to the downstream commands called by the plugin. Then I think I definitely need…
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