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Forum: Site Use Guidelines
meta biostars Guidelines
updated 2 hours ago by 43k • written 14.6 years ago by 100k
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Forum: Ideal PC configurations and operating system for bioinformatics laboratory
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Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
16 hours ago by yxwucq • 0
3 results • Page 1 of 1
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Comment: Ideal PC configurations and operating system for bioinformatics laboratory
C: Threshold over percentage identity in blastn
A: Threshold over percentage identity in blastn
The Biostar Herald for Tuesday, April 23, 2024
Answer: Including plasmid in transcriptome assemblies
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
RNA-seq power estimation using ssizeRNA program
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Comment: TCGA2STAT Error: Firehose connection by Ram 43k
If their github repo is alive, raise an issue there.
Comment: TCGA2STAT Error: Firehose connection by Ngrin • 0
Yeah makes sense. I was following their github. Thanks for pointing this out.
Comment: The Biostar Herald for Tuesday, April 23, 2024 by Pierre Lindenbaum 161k
bwa 0.7.18 released ! https://github.com/lh3/bwa/releases/tag/v0.7.18
Comment: Downsampling fastq file by Ram 43k
I'm moving this to a comment for now.
Answer: Seurat merge and batch correction by bk11 ★ 2.3k
You can merge your seven Seurat objects and perform integration using **Seurat V5** to remove the batch effect- ```r library(Seurat) libra…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie by atowns21 • 0
Yeah, I've been digging around the web for a while now. Can't find any information about the barcodes. The total number of combinations is …
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie by GenoMax 141k
Ah I see that in now. I am not sure why MGI has split the whitelist into two sections. You may need to see if you can dig up some info abou…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie by atowns21 • 0
read 1 is 30bp... which I think you meant to type. If you scroll approximately halfway down the json file I linked to, it has the following…
Comment: TCGA2STAT Error: Firehose connection by Ram 43k
The package has been removed from CRAN and BioConductor. Should you even be using it?
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie by GenoMax 141k
The barcodes are in location 1-10 and you have the actual list there. I don't know for certain what is in position 11-20 (perhaps spacer, c…
Answer: Including plasmid in transcriptome assemblies by GenoMax 141k
> I want to use both STAR and BOWTIE2 for assembly Those programs are aligners not assemblers. But since you are working with bacteria yo…
Comment: How to slice a gvcf file with tabix? by Sd • 0
I already have gvcfs called by chromosome and there are 24 gvcfs per sample which I merged them into a single gvcf per sample. Now I want …
Comment: Find subcluster under a cluster, find differential genes in one cluster between by bk11 ★ 2.3k
First, could you please explain any reason for not using Seurat V5 approach for your data integration mentioned in [here][1]? > Now moving…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie by atowns21 • 0
So I'd take the json file located [here][1] and essentially create the whitelist to be all possible combinations of the position 1-10 barco…
Comment: TCGA2STAT Error: Firehose connection by Ngrin • 0
Hey Gnana, could you find a way to solve the issue? I have the same error
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