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1,000 results • Page
2 of 20
Sort: Views
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Views
Votes
Replies
8
votes
9
replies
10k
views
Forum:
How to calculate FPKM values of interested gene
cufflinks
FPKM
bowtie2
RNA-Seq
updated 19 months ago by
Ram
39k • written 8.9 years ago by
Ginsea Chen
▴ 130
32
votes
16
replies
10k
views
9 follow
Forum:
How to switch from bench research to computational biology?
career
updated 11 months ago by
Ram
39k • written 8.2 years ago by
yuggnurug
▴ 70
32
votes
13
replies
10k
views
10 follow
Forum:
What Skills Do You Have To Have As A Phd Graduate In Bioinformatics?
genomics
phd
updated 3 months ago by
Ram
39k • written 10.8 years ago by
sentausa
▴ 650
12
votes
8
replies
10k
views
8 follow
Forum:
"Separate enrichment analysis of pathways for up- and downregulated genes"
enrichmnet-analysis
updated 12 weeks ago by
Ram
39k • written 9.1 years ago by
Woa
★ 2.9k
37
votes
21
replies
10.0k
views
10 follow
Forum:
Orientation of PE reads a review of --fr --ff and --rf meanings
RNA-Seq
updated 5 days ago by
Ram
39k • written 4.6 years ago by
Juke34
7.9k
15
votes
14
replies
9.9k
views
6 follow
Forum:
How Should I Go About Preparing For My Bioinformatics Phd Admissions Interview?
phd
updated 12 weeks ago by
Ram
39k • written 9.3 years ago by
kandoigaurav
▴ 150
15
votes
6
replies
9.7k
views
Forum:
Bioinformatics short paper journal resource [software/webapps/database]
journal
software
webapp
updated 7 weeks ago by
Ram
39k • written 7.1 years ago by
Sukhi Singh
11k
3
votes
2
replies
9.6k
views
Forum:
How To Provide Reference Sequence Dictionary To Reordersam?
picard
gatk
updated 3 months ago by
Ram
39k • written 9.5 years ago by
Charles Warden
8.2k
12
votes
6
replies
9.6k
views
Forum:
Looking for opinions on PhD programs in Genetics and Genomics/Bioinformatics
PhD
Genomics
updated 15 months ago by
Ram
39k • written 8.6 years ago by
Kssr
▴ 110
144
votes
62
replies
9.6k
views
23 follow
Forum:
Is The Biostar Fading? (Updated For 2014)
meta
biostars
updated 3 months ago by
Ram
39k • written 12.2 years ago by
Joachim
★ 2.9k
24
votes
15
replies
9.5k
views
11 follow
Forum:
PhD in bioinformatics or biostatistics as a wet-lab scientist?
biology
statistics
PhD
updated 11 weeks ago by
Ram
39k • written 8.7 years ago by
benjamin.hebisch
▴ 70
10
votes
6
replies
9.5k
views
Forum:
Is it worth getting a Masters in Bioinformatics, financially?
career
updated 12 weeks ago by
Ram
39k • written 9.0 years ago by
n00514450
▴ 20
37
votes
19
replies
9.5k
views
10 follow
Forum:
Kallisto New RNA-seq quantification method discussion
RNA-Seq
updated 7 weeks ago by
Ram
39k • written 7.1 years ago by
morovatunc
▴ 540
34
votes
12
replies
9.5k
views
9 follow
Forum:
Is going back to the wet lab worth it?
careerwet-lab
updated 6 weeks ago by
Ram
39k • written 6.4 years ago by
ropolocan
▴ 760
6
votes
5
replies
9.5k
views
Forum:
Single-end sequencing versus paired-end
sequencing
single versus paired
Assembly
snp
Forum
3.3 years ago by
Sujata
▴ 20
0
votes
2
replies
9.4k
views
Forum:
Secondary Structure Prediction software RNA/DNA
dna
secondary-structure
software
rna
updated 11 weeks ago by
Ram
39k • written 8.5 years ago by
14134125465346445
★ 3.6k
50
votes
8
replies
9.1k
views
6 follow
Forum:
Encode Commentary From Dan Graur
encode
updated 3 months ago by
Ram
39k • written 10.3 years ago by
Damian Kao
16k
5
votes
4
replies
9.1k
views
Forum:
List of unresolved problems in Computational Biology / Bioinformatics?
computational-biology
problems
updated 11 weeks ago by
Ram
39k • written 8.4 years ago by
Samuel Lampa
★ 1.3k
11
votes
12
replies
9.0k
views
Forum:
need ideas for bioinformatics research project (undergraduates)
alignment
sequence
updated 13 months ago by
Ram
39k • written 8.3 years ago by
fzrh
▴ 10
86
votes
29
replies
8.7k
views
9 follow
Forum:
What is the future of EMBL-EBI (and European bioinformatics in general) in case of a 'Brexit'?
EU
brexit
EBI
politics
updated 7 weeks ago by
Ram
39k • written 7.0 years ago by
Michael
52k
11
votes
10
replies
8.7k
views
Forum:
Looking For Bioinformatics Job In Germany
bioinformatician
career
updated 12 weeks ago by
Ram
39k • written 9.2 years ago by
jack
▴ 520
29
votes
10
replies
8.7k
views
Forum:
Principles of Tidy Data (Hadley Wickham) and the VCF format
vcf
tidy-data
updated 15 months ago by
Ram
39k • written 8.5 years ago by
Giovanni M Dall'Olio
27k
51
votes
24
replies
8.4k
views
7 follow
Forum:
Bio-informatics salary distribution versus Java salary distribution in the UK
career
updated 17 months ago by
Ram
39k • written 8.8 years ago by
William
★ 5.2k
88
votes
32
replies
8.3k
views
11 follow
Forum:
Vintage / unconventional pictures for Bioinformatics
history
picture
updated 14 months ago by
Ram
39k • written 8.6 years ago by
Pierre Lindenbaum
154k
67
votes
40
replies
8.3k
views
12 follow
Forum:
I want to re-open the old debate: python or perl ?
perl
python
updated 15 months ago by
Ram
39k • written 8.5 years ago by
Gabriel R.
★ 2.9k
12
votes
4
replies
8.3k
views
Forum:
Bioinformatics/Biotech Firms In India
career
updated 3 months ago by
Ram
39k • written 11.1 years ago by
aj123
▴ 100
4
votes
5
replies
8.3k
views
Forum:
Computer Science's Perspective Comparison : BLAST vs. Clustal Omega ?!
clustal-omega
blast
updated 12 months ago by
Ram
39k • written 8.2 years ago by
Bara'a
▴ 270
34
votes
11
replies
8.2k
views
13 follow
Forum:
Gallery: Computational tools for DNA methylation
computational-tools
methylation
updated 9 months ago by
Ram
39k • written 7.6 years ago by
Shicheng Guo
★ 9.3k
13
votes
7
replies
8.2k
views
Forum:
The struggle between fastq and fastq.gz, compressed v/s uncompressed file formats
fastq
updated 18 days ago by
Ram
39k • written 5.9 years ago by
lakhujanivijay
5.7k
22
votes
14
replies
8.2k
views
6 follow
Forum:
Too late to switch to Bioinformatics?
education
career
updated 12 weeks ago by
Ram
39k • written 9.0 years ago by
taneuroth
▴ 30
49
votes
4
replies
8.1k
views
Forum:
How to add images to a Biostars post
how-to
3 months ago by
Ram
39k
10
votes
3
replies
8.1k
views
Forum:
Got Multiple Cpus? Activate Them With Gnu Parallel
parallel
command-line
ngs
updated 3 months ago by
Ram
39k • written 9.5 years ago by
ole.tange
★ 4.3k
244
votes
36
replies
8.0k
views
32 follow
Forum:
Good Habit for Bioinformatics Analyst or Scientist
Analyst
Scientist
updated 7 weeks ago by
Ram
39k • written 7.1 years ago by
Shicheng Guo
★ 9.3k
79
votes
35
replies
8.0k
views
17 follow
Forum:
Do bioinformaticians often break molecular biologists' hearts by being the first author?
authorship
career
updated 2 days ago by
jli.ww
• 0 • written 7.5 years ago by
sentausa
▴ 650
99
votes
21
replies
8.0k
views
14 follow
Forum:
How Has Bioinformatics Improved Over Time?
bioinformatics
updated 3 months ago by
Ram
39k • written 10.3 years ago by
Kate
▴ 370
9
votes
4
replies
8.0k
views
Forum:
Biofinysics - How does bowtie2 assign MAPQ scores?
alignment
bowtie2
updated 11 weeks ago by
Ram
39k • written 8.8 years ago by
Philipp Bayer
7.8k
21
votes
17
replies
7.9k
views
7 follow
Forum:
In YOUR personal opinion, who are the top 5 bioinformatics researchers in NGS in 21st century?
next-gen
updated 6 weeks ago by
Ram
39k • written 6.4 years ago by
AptRank
▴ 10
9
votes
19
replies
7.9k
views
Forum:
Careful!! Velvet Generates Different Assemblies From The Same Input Data And Same Input Parameters With Openmp Enabled.
velvet
genome
updated 3 months ago by
Ram
39k • written 9.5 years ago by
Rahul Sharma
▴ 650
13
votes
5
replies
7.9k
views
Forum:
Funding Opportunities In Bioinformatics
grant
updated 3 months ago by
Ram
39k • written 10.3 years ago by
Obi Griffith
20k
27
votes
21
replies
7.9k
views
9 follow
Forum:
Better for my career: Degree in computer science or Phd in bioinformatics?
computer-science
phd
updated 7 weeks ago by
Ram
39k • written 6.7 years ago by
BioinfGuru
★ 1.6k
46
votes
9
replies
7.9k
views
10 follow
Forum:
Bioinformatics For Clinicians - What To Teach Them?
education
updated 12 weeks ago by
Ram
39k • written 9.5 years ago by
j
▴ 140
64
votes
8
replies
7.8k
views
7 follow
Forum:
Isaac Asimov Accurately Predicts Woes Of Bioinformatician
bioinformatician
updated 3 months ago by
Ram
39k • written 10.1 years ago by
Obi Griffith
20k
26
votes
0
replies
7.8k
views
Forum:
Heng Li of BWA and Samtools uses this
uses-this
updated 11 weeks ago by
Ram
39k • written 8.6 years ago by
Istvan Albert
98k
19
votes
9
replies
7.7k
views
6 follow
Forum:
How to support bioinformatics tools via Biostars: prefill and watch tags, reply via email
biostars
updated 3 months ago by
Ram
39k • written 9.1 years ago by
Istvan Albert
98k
4
votes
6
replies
7.7k
views
Forum:
TCGA: How to tell if the data is exome or WGS?
wgs
mutations
tcga
exome
updated 7 months ago by
Ram
39k • written 7.8 years ago by
Les Ander
▴ 110
3
votes
5
replies
7.5k
views
Forum:
PopGenome nucleotide diversity
next-gen
SNP
R
gene
updated 8 weeks ago by
Ram
39k • written 7.2 years ago by
yp
▴ 10
145
votes
56
replies
7.5k
views
17 follow
Forum:
Suggesting The Removal Of Post Closing
biostars
updated 3 months ago by
Ram
39k • written 10.2 years ago by
Istvan Albert
98k
49
votes
36
replies
7.4k
views
11 follow
Forum:
New features and bug fixes for Biostar to work on during Google Summer of Code
meta
biostars
gsoc
updated 3 months ago by
Ram
39k • written 9.1 years ago by
Paolo
▴ 110
47
votes
37
replies
7.4k
views
12 follow
Forum:
The Organisation Of Really Contrived Acronyms (Orca)
acronyms
updated 3 months ago by
Ram
39k • written 9.8 years ago by
Niallhaslam
2.3k
28
votes
14
replies
7.4k
views
8 follow
Forum:
Should I work first in a Bioinformatics company or get a Ph. D.?
Biochemistry
PhD
career
updated 7 weeks ago by
Ram
39k • written 7.1 years ago by
mvasquez16
▴ 20
1,000 results • Page
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Recent Votes
Answer: How do you add an ORF that overlaps the two regions where a circular genome is c
C: PLINK logistic regression analysis and covariates
Comment: Regular Expression for conversion
Answer: RNAseq Data and Pipeline
Comment: Salmon Index
Comment: Salmon Index
Resequencing data of pangenome
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Recent Replies
Comment: Unusual FASTQ file
by
Ram
39k
People from the middle east/indian subcontinent often associate the term "post title" with their job title, because a) post translates more…
Comment: How to make a dotplot for bulk RNA average expression ?
by
bhumm
▴ 40
I am not entirely clear on what you are trying to accomplish, but based on @Trivas answer, I would first transform the data relative to ave…
Answer: What is the best way to clean bulk RNA-seq data?
by
swbarnes2
13k
There really is no "best". Just find something reasonable, and document what you chose.
Comment: How to deal with duplicated gene IDs in TCGA RNA expression data?
by
Vincent Laufer
★ 2.8k
ok interesting. Do all, most, some, or a few of the genes have repeated lines. Is there a handy link to the exact file you are looking at?
Answer: Make a BedGraph file
by
bhumm
▴ 40
Bedtools window piped into genomecov? https://bedtools.readthedocs.io/en/latest/content/tools/window.html https://bedtools.readthedocs.io…
Answer: RNAseq Data and Pipeline
by
ATpoint
72k
STAR counts are the raw counts, that is appropriate for DESeq2.
Comment: RNAseq Data and Pipeline
by
swbarnes2
13k
STAR has a GeneCounts output that looks as you describe; three columns, depending on whether one thinks the prep is unstranded, or stranded…
Comment: RNAseq Data and Pipeline
by
Ram
39k
There is at least one more step that you haven't shared, isn't there? This line alone does not get you the data. You don't need to give us …
Comment: RNAseq Data and Pipeline
by
turcoa1
• 0
my entire code is not done. All I am trying to understand is if the STAR - counts downloaded from this query have raw reads. There are head…
Comment: RNAseq Data and Pipeline
by
Ram
39k
Please provide your entire code so those of us wishing to do a quick test can download and check for ourselves. Also, please use the format…
Comment: RNAseq Data and Pipeline
by
turcoa1
• 0
```r query <- GDCquery( project = "TCGA-THCA", data.category = "Transcriptome Profiling", data.type = "Gene Expression Quantificatio…
Comment: NCBI Common Taxonomy Tree
by
GenoMax
129k
It may be best to email NCBI help desk with these questions. Post their response here once you hear back from them.
Comment: RNAseq Data and Pipeline
by
Ram
39k
What data are you downloading? Can you show us your GDCquery commands please?
Comment: Salmon Index
by
ashleymb1116
• 0
I am working on cow, Bos Taurus. The Arabidopsis code was an example code that was on Salmon's github and an example for indexing they gave…
Comment: Salmon Index
by
GenoMax
129k
> I am using the Bos Taurus files Which organism are you actually working on? You have mentioned three so far, arabidopsis, human and now …
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